Large extracellular vesicles transfer more prions and infect cell culture better than small extracellular vesicles
Identifikátory výsledku
Kód výsledku v IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11110%2F23%3A10472616" target="_blank" >RIV/00216208:11110/23:10472616 - isvavai.cz</a>
Nalezeny alternativní kódy
RIV/00216208:11310/23:10472616
Výsledek na webu
<a href="https://verso.is.cuni.cz/pub/verso.fpl?fname=obd_publikace_handle&handle=2v3Lr7aiz0" target="_blank" >https://verso.is.cuni.cz/pub/verso.fpl?fname=obd_publikace_handle&handle=2v3Lr7aiz0</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.bbrc.2023.149208" target="_blank" >10.1016/j.bbrc.2023.149208</a>
Alternativní jazyky
Jazyk výsledku
angličtina
Název v původním jazyce
Large extracellular vesicles transfer more prions and infect cell culture better than small extracellular vesicles
Popis výsledku v původním jazyce
Prions are responsible for a number of lethal neurodegenerative and transmissible diseases in humans and animals. Extracellular vesicles, especially small exosomes, have been extensively studied in connection with various diseases. In contrast, larger microvesicles are often overlooked. In this work, we compared the ability of large extracellular vesicles (lEVs) and small extracellular vesicles (sEVs) to spread prions in cell culture. We utilized CAD5 cell culture model of prion infection and isolated lEVs by 20,000xg force and sEVs by 110,000xg force. The lEV fraction was enriched in beta-1 integrin with a vesicle size starting at 100 nm. The fraction of sEVs was partially depleted of beta-1 integrin with a mean size of 79 nm. Both fractions were enriched in prion protein, but the lEVs contained a higher prion-converting activity. In addition, lEV infection led to stronger prion signals in both cell cultures, as detected by cell and western blotting. These results were verified on N2a-PK1 cell culture. Our data suggest the importance of lEVs in the trafficking and spread of prions over extensively studied small EVs.
Název v anglickém jazyce
Large extracellular vesicles transfer more prions and infect cell culture better than small extracellular vesicles
Popis výsledku anglicky
Prions are responsible for a number of lethal neurodegenerative and transmissible diseases in humans and animals. Extracellular vesicles, especially small exosomes, have been extensively studied in connection with various diseases. In contrast, larger microvesicles are often overlooked. In this work, we compared the ability of large extracellular vesicles (lEVs) and small extracellular vesicles (sEVs) to spread prions in cell culture. We utilized CAD5 cell culture model of prion infection and isolated lEVs by 20,000xg force and sEVs by 110,000xg force. The lEV fraction was enriched in beta-1 integrin with a vesicle size starting at 100 nm. The fraction of sEVs was partially depleted of beta-1 integrin with a mean size of 79 nm. Both fractions were enriched in prion protein, but the lEVs contained a higher prion-converting activity. In addition, lEV infection led to stronger prion signals in both cell cultures, as detected by cell and western blotting. These results were verified on N2a-PK1 cell culture. Our data suggest the importance of lEVs in the trafficking and spread of prions over extensively studied small EVs.
Klasifikace
Druh
J<sub>imp</sub> - Článek v periodiku v databázi Web of Science
CEP obor
—
OECD FORD obor
30303 - Infectious Diseases
Návaznosti výsledku
Projekt
<a href="/cs/project/LX22NPO5107" target="_blank" >LX22NPO5107: Národní ústav pro neurologický výzkum</a><br>
Návaznosti
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>S - Specificky vyzkum na vysokych skolach<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Ostatní
Rok uplatnění
2023
Kód důvěrnosti údajů
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Údaje specifické pro druh výsledku
Název periodika
Biochemical and Biophysical Research Communications
ISSN
0006-291X
e-ISSN
1090-2104
Svazek periodika
687
Číslo periodika v rámci svazku
December
Stát vydavatele periodika
US - Spojené státy americké
Počet stran výsledku
15
Strana od-do
149208
Kód UT WoS článku
001112273600001
EID výsledku v databázi Scopus
2-s2.0-85176128201