The effect of aldehyde fixatives, alcohol-based fixatives, and fixatives combining denaturation and crosslinking on histomorphological, histochemical, and molecular genetic studies during short-term and long-term fixation: a comprehensive analysis

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11110%2F24%3A10486051" target="_blank" >RIV/00216208:11110/24:10486051 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00216208:11140/24:10486051 RIV/62157124:16170/24:43881225

Výsledek na webu

<a href="https://verso.is.cuni.cz/pub/verso.fpl?fname=obd_publikace_handle&handle=UCQ.P.5lPN" target="_blank" >https://verso.is.cuni.cz/pub/verso.fpl?fname=obd_publikace_handle&handle=UCQ.P.5lPN</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.2754/avb202493030321" target="_blank" >10.2754/avb202493030321</a>

Jazyk výsledku

angličtina

Název v původním jazyce

The effect of aldehyde fixatives, alcohol-based fixatives, and fixatives combining denaturation and crosslinking on histomorphological, histochemical, and molecular genetic studies during short-term and long-term fixation: a comprehensive analysis

Popis výsledku v původním jazyce

The choice of fixative solution has a significant impact on the quality of tissue processing and the results of ancillary pathological studies. The present study aimed to compare the effect of different fixatives and fixative times on routinely used histopathological staining methods and molecular genetic studies in porcine endometrium. Haematoxylin and eosin staining was used to evaluate cell morphology, while Gömöri and orcein staining were selected for histochemical studies; molecular genetic studies were represented by polymerase chain reaction. Tissue samples were fixed in formalin, Bouin&apos;s fluid, methacarn, Greenfix, and Antigenfix for 1.5; 8.5; 15.5; 29.5; 64.5; 189.5; 249.5; 309.5; 369.5 days (only the period 1.5 days to 189.5 days was analyzed for histochemical studies). Formol proved to be the best for preserving cell morphology during both short-term and long-term fixation. Alcohol fixatives mainly affected the nuclear morphology, therefore they are not completely suitable for histomorphological analysis. The best preservation of nuclear morphology was achieved with Bouin&apos;s fixative. For histochemical studies and shortterm fixation, fixative solutions of all 3 groups (crosslinking, denaturing fixatives and fixatives combining both denaturing and cross-linking effects) were suitable. For long-term fixation, the alcohol fixative methacarn proved to be the best in histochemical studies. Finally, for molecular genetic studies, methacarn was the best fixative, while Bouin&apos;s fluid was completely unsuitable. Formalin was confirmed as the most universal fixative overall. However, the selection of a suitable fixative should always be made depending on the specific pathological study and the f ixation time.

Název v anglickém jazyce

The effect of aldehyde fixatives, alcohol-based fixatives, and fixatives combining denaturation and crosslinking on histomorphological, histochemical, and molecular genetic studies during short-term and long-term fixation: a comprehensive analysis

Popis výsledku anglicky

The choice of fixative solution has a significant impact on the quality of tissue processing and the results of ancillary pathological studies. The present study aimed to compare the effect of different fixatives and fixative times on routinely used histopathological staining methods and molecular genetic studies in porcine endometrium. Haematoxylin and eosin staining was used to evaluate cell morphology, while Gömöri and orcein staining were selected for histochemical studies; molecular genetic studies were represented by polymerase chain reaction. Tissue samples were fixed in formalin, Bouin&apos;s fluid, methacarn, Greenfix, and Antigenfix for 1.5; 8.5; 15.5; 29.5; 64.5; 189.5; 249.5; 309.5; 369.5 days (only the period 1.5 days to 189.5 days was analyzed for histochemical studies). Formol proved to be the best for preserving cell morphology during both short-term and long-term fixation. Alcohol fixatives mainly affected the nuclear morphology, therefore they are not completely suitable for histomorphological analysis. The best preservation of nuclear morphology was achieved with Bouin&apos;s fixative. For histochemical studies and shortterm fixation, fixative solutions of all 3 groups (crosslinking, denaturing fixatives and fixatives combining both denaturing and cross-linking effects) were suitable. For long-term fixation, the alcohol fixative methacarn proved to be the best in histochemical studies. Finally, for molecular genetic studies, methacarn was the best fixative, while Bouin&apos;s fluid was completely unsuitable. Formalin was confirmed as the most universal fixative overall. However, the selection of a suitable fixative should always be made depending on the specific pathological study and the f ixation time.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

30109 - Pathology

Projekt

—

Návaznosti

V - Vyzkumna aktivita podporovana z jinych verejnych zdroju

Rok uplatnění

2024

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Acta Veterinaria Brno

ISSN

0001-7213

e-ISSN

1801-7576

Svazek periodika

93

Číslo periodika v rámci svazku

3

Stát vydavatele periodika

CZ - Česká republika

Počet stran výsledku

10

Strana od-do

321-330

Kód UT WoS článku

001338130400009

EID výsledku v databázi Scopus

2-s2.0-85206896623