Cyclin A down-regulation in TGF beta 1-arrested follicular lymphoma cells
Identifikátory výsledku
Kód výsledku v IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11120%2F00%3A00003750" target="_blank" >RIV/00216208:11120/00:00003750 - isvavai.cz</a>
Výsledek na webu
<a href="http://dx.doi.org/10.1006/excr.2000.5047" target="_blank" >http://dx.doi.org/10.1006/excr.2000.5047</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1006/excr.2000.5047" target="_blank" >10.1006/excr.2000.5047</a>
Alternativní jazyky
Jazyk výsledku
angličtina
Název v původním jazyce
Cyclin A down-regulation in TGF beta 1-arrested follicular lymphoma cells
Popis výsledku v původním jazyce
Transforming growth factor beta1 (TGF beta1) induces growth arrest in many cell types, including B lymphocytes. We examined the effect of TGF on cell cycle progression of a non-Hodgkin lymphoma cell line of follicular lymphoma subtype (FL). After 48 h ofTGF beta1 (10 ng/ml) treatment, a significantly increased number of DoHH2 cells was retained in G(0)/G(1) phase. We examined the level of cell cycle components, cyclins, cyclin-dependent kinases (cdk), and their inhibitors. We found that the expressionof cyclin A and p21(WAF1) molecules was primarily modulated by TGF beta1 treatment while the expression of other regulatory components, like cyclins D, cyclin E, cdk2, cdk4, and cdk6 or p15(INK4B), p16(INK4A), and p27(KIP1) was not significantly affected. We further examined expression and activity of CREB/ATF family members to examine their roles in cyclin A inhibition. The binding activity of CREB-1 and ATF-8 to the CRE region of the cyclin A promoter was almost completely abolished du
Název v anglickém jazyce
Cyclin A down-regulation in TGF beta 1-arrested follicular lymphoma cells
Popis výsledku anglicky
Transforming growth factor beta1 (TGF beta1) induces growth arrest in many cell types, including B lymphocytes. We examined the effect of TGF on cell cycle progression of a non-Hodgkin lymphoma cell line of follicular lymphoma subtype (FL). After 48 h ofTGF beta1 (10 ng/ml) treatment, a significantly increased number of DoHH2 cells was retained in G(0)/G(1) phase. We examined the level of cell cycle components, cyclins, cyclin-dependent kinases (cdk), and their inhibitors. We found that the expressionof cyclin A and p21(WAF1) molecules was primarily modulated by TGF beta1 treatment while the expression of other regulatory components, like cyclins D, cyclin E, cdk2, cdk4, and cdk6 or p15(INK4B), p16(INK4A), and p27(KIP1) was not significantly affected. We further examined expression and activity of CREB/ATF family members to examine their roles in cyclin A inhibition. The binding activity of CREB-1 and ATF-8 to the CRE region of the cyclin A promoter was almost completely abolished du
Klasifikace
Druh
J<sub>x</sub> - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)
CEP obor
FD - Onkologie a hematologie
OECD FORD obor
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Návaznosti výsledku
Projekt
<a href="/cs/project/VS96129" target="_blank" >VS96129: Laboratoř genové exprese</a><br>
Návaznosti
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Ostatní
Rok uplatnění
2000
Kód důvěrnosti údajů
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Údaje specifické pro druh výsledku
Název periodika
Experimental Cell Research
ISSN
0014-4827
e-ISSN
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Svazek periodika
261
Číslo periodika v rámci svazku
1
Stát vydavatele periodika
US - Spojené státy americké
Počet stran výsledku
10
Strana od-do
250-259
Kód UT WoS článku
000165622200027
EID výsledku v databázi Scopus
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