Testicular Sperm Aneuploidy in Nonobstructive Azoospermic Patients

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11120%2F12%3A43902226" target="_blank" >RIV/00216208:11120/12:43902226 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00027162:_____/12:#0000890 RIV/00064173:_____/12:43902226

Výsledek na webu

<a href="http://dx.doi.org/10.1093/humrep/des115" target="_blank" >http://dx.doi.org/10.1093/humrep/des115</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1093/humrep/des115" target="_blank" >10.1093/humrep/des115</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Testicular Sperm Aneuploidy in Nonobstructive Azoospermic Patients

Popis výsledku v původním jazyce

BACKGROUND: Nonobstructive azoospermic (NOA) men can father children after testicular sperm extraction (TESE). They were shown to be at risk of producing chromosomally abnormal spermatozoa, but the number of sperm analysed per patient is usually very low. METHODS: Multicolor fluorescence in situ hybridization was used for detection of chromosome 13, 15, 16, 18, 21, 22, X and Y disomy and diploidy in sperm obtained from NOA men (n=17) and control donors (n=10). At least 500 testicular sperm were scored in each patient to increase the predictability rate of our study. RESULTS: The mean frequency of overall disomy (2.32%) and diploidy (0.80%) found in 13,689 testicular spermatozoa of NOA patients was significantly higher than in the ejaculated sperm of normospermic control donors (0.62% of disomy and 0.29% of diploidy). A highly significant increase in frequencies of chromosome 15, Y and overall disomy (P<0.001), and a significant increase in disomy of chromosome 13 (P=0.002), 16 (P=0.031

Název v anglickém jazyce

Testicular Sperm Aneuploidy in Nonobstructive Azoospermic Patients

Popis výsledku anglicky

BACKGROUND: Nonobstructive azoospermic (NOA) men can father children after testicular sperm extraction (TESE). They were shown to be at risk of producing chromosomally abnormal spermatozoa, but the number of sperm analysed per patient is usually very low. METHODS: Multicolor fluorescence in situ hybridization was used for detection of chromosome 13, 15, 16, 18, 21, 22, X and Y disomy and diploidy in sperm obtained from NOA men (n=17) and control donors (n=10). At least 500 testicular sperm were scored in each patient to increase the predictability rate of our study. RESULTS: The mean frequency of overall disomy (2.32%) and diploidy (0.80%) found in 13,689 testicular spermatozoa of NOA patients was significantly higher than in the ejaculated sperm of normospermic control donors (0.62% of disomy and 0.29% of diploidy). A highly significant increase in frequencies of chromosome 15, Y and overall disomy (P<0.001), and a significant increase in disomy of chromosome 13 (P=0.002), 16 (P=0.031

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

EB - Genetika a molekulární biologie

OECD FORD obor

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Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

S - Specificky vyzkum na vysokych skolach

Rok uplatnění

2012

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Human Reproduction

ISSN

0268-1161

e-ISSN

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Svazek periodika

27

Číslo periodika v rámci svazku

7

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

8

Strana od-do

2233-2239

Kód UT WoS článku

000305458800039

EID výsledku v databázi Scopus

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