NT5E CpG island methylation is a favourable breast cancer biomarker

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11120%2F12%3A43906998" target="_blank" >RIV/00216208:11120/12:43906998 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00064173:_____/12:43906998

Výsledek na webu

<a href="http://dx.doi.org/10.1038/bjc.2012.212" target="_blank" >http://dx.doi.org/10.1038/bjc.2012.212</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1038/bjc.2012.212" target="_blank" >10.1038/bjc.2012.212</a>

Jazyk výsledku

angličtina

Název v původním jazyce

NT5E CpG island methylation is a favourable breast cancer biomarker

Popis výsledku v původním jazyce

Relapse risk assessment and individual treatment recommendations remain suboptimal for breast cancer patients. In the light of existing preclinical and clinical data, we studied NT5E (5'-nucleotidase, ecto) expression and NT5E CpG island methylation in breast cancer. METHODS: We used RT-PCR, qPCR, methylation-specific PCR and pyrosequencing to analyse NT5E in breast carcinoma cell lines and primary and breast carcinomas. RESULTS: NT5E CpG island methylation was inversely associated with NT5E expressionin breast carcinoma cell lines. In clinical series, patients whose primary tumours had NT5E CpG island methylation were less likely to develop metastasis (P = 0.003, OR = 0.34, 95% CI: 0.17-0.69). In 3/4 paired samples, NT5E was methylated in primary tumours and demethylated in CNS metastases. Patients progressing to non-visceral as compared with visceral metastases were more likely to have NT5E CpG island methylation in primary tumours (P = 0.01, OR = 11.8). Patients with tumours lackin

Název v anglickém jazyce

NT5E CpG island methylation is a favourable breast cancer biomarker

Popis výsledku anglicky

Relapse risk assessment and individual treatment recommendations remain suboptimal for breast cancer patients. In the light of existing preclinical and clinical data, we studied NT5E (5'-nucleotidase, ecto) expression and NT5E CpG island methylation in breast cancer. METHODS: We used RT-PCR, qPCR, methylation-specific PCR and pyrosequencing to analyse NT5E in breast carcinoma cell lines and primary and breast carcinomas. RESULTS: NT5E CpG island methylation was inversely associated with NT5E expressionin breast carcinoma cell lines. In clinical series, patients whose primary tumours had NT5E CpG island methylation were less likely to develop metastasis (P = 0.003, OR = 0.34, 95% CI: 0.17-0.69). In 3/4 paired samples, NT5E was methylated in primary tumours and demethylated in CNS metastases. Patients progressing to non-visceral as compared with visceral metastases were more likely to have NT5E CpG island methylation in primary tumours (P = 0.01, OR = 11.8). Patients with tumours lackin

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

FD - Onkologie a hematologie

OECD FORD obor

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Projekt

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Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2012

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

British Journal of Cancer

ISSN

0007-0920

e-ISSN

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Svazek periodika

107

Číslo periodika v rámci svazku

1

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

9

Strana od-do

75-83

Kód UT WoS článku

000305888400012

EID výsledku v databázi Scopus

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