Large Volume Sample Stacking for Rapid and Sensitive Determination of Antidiabetic Drug Metformin in Human Urine and Serum by Capillary Electrophoresis with Contactless Conductivity Detection
Identifikátory výsledku
Kód výsledku v IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11120%2F14%3A43908436" target="_blank" >RIV/00216208:11120/14:43908436 - isvavai.cz</a>
Výsledek na webu
<a href="http://dx.doi.org/10.1016/j.chroma.2014.04.016" target="_blank" >http://dx.doi.org/10.1016/j.chroma.2014.04.016</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.chroma.2014.04.016" target="_blank" >10.1016/j.chroma.2014.04.016</a>
Alternativní jazyky
Jazyk výsledku
angličtina
Název v původním jazyce
Large Volume Sample Stacking for Rapid and Sensitive Determination of Antidiabetic Drug Metformin in Human Urine and Serum by Capillary Electrophoresis with Contactless Conductivity Detection
Popis výsledku v původním jazyce
Two CE methods with contactless conductivity detection have been developed for determining the oral antidiabetic drug metformin in human urine and blood. The determination of metformin is performed on a separation capillary with an effective length of 14cm, using a maximum voltage of 30 kV and with a small injection of 50-fold diluted urine into the capillary. Under these conditions, the migration time of metformin is 35 s and the LOD is 0.3 ?M. Large-volume sample stacking was used to determine low metformin levels in serum. The injection of a sample of serum deproteinized with acetonitrile was 10 times greater compared to the injected amount of urine. This enabled reduction of the LOD to 0.03 ?M and the metformin migration time equalled 86 s. The undesirable solvent from sample zone was forced out of the capillary to ensure rapidity and good repeatability of the determination. The RSD values for the migration time are 0.1 % for urine and 0.7 % for serum; RSD for the peak areas equal
Název v anglickém jazyce
Large Volume Sample Stacking for Rapid and Sensitive Determination of Antidiabetic Drug Metformin in Human Urine and Serum by Capillary Electrophoresis with Contactless Conductivity Detection
Popis výsledku anglicky
Two CE methods with contactless conductivity detection have been developed for determining the oral antidiabetic drug metformin in human urine and blood. The determination of metformin is performed on a separation capillary with an effective length of 14cm, using a maximum voltage of 30 kV and with a small injection of 50-fold diluted urine into the capillary. Under these conditions, the migration time of metformin is 35 s and the LOD is 0.3 ?M. Large-volume sample stacking was used to determine low metformin levels in serum. The injection of a sample of serum deproteinized with acetonitrile was 10 times greater compared to the injected amount of urine. This enabled reduction of the LOD to 0.03 ?M and the metformin migration time equalled 86 s. The undesirable solvent from sample zone was forced out of the capillary to ensure rapidity and good repeatability of the determination. The RSD values for the migration time are 0.1 % for urine and 0.7 % for serum; RSD for the peak areas equal
Klasifikace
Druh
J<sub>x</sub> - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)
CEP obor
CB - Analytická chemie, separace
OECD FORD obor
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Návaznosti výsledku
Projekt
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Návaznosti
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Ostatní
Rok uplatnění
2014
Kód důvěrnosti údajů
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Údaje specifické pro druh výsledku
Název periodika
Journal of Chromatography A
ISSN
0021-9673
e-ISSN
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Svazek periodika
1345
Číslo periodika v rámci svazku
June 6
Stát vydavatele periodika
NL - Nizozemsko
Počet stran výsledku
5
Strana od-do
207-211
Kód UT WoS článku
000336709300023
EID výsledku v databázi Scopus
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