Circulating tumour cells in patients with urothelial tumours: Enrichment and in vitro culture

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11120%2F14%3A43909166" target="_blank" >RIV/00216208:11120/14:43909166 - isvavai.cz</a>

Výsledek na webu

<a href="http://dx.doi.org/10.5489/cuaj.1978" target="_blank" >http://dx.doi.org/10.5489/cuaj.1978</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.5489/cuaj.1978" target="_blank" >10.5489/cuaj.1978</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Circulating tumour cells in patients with urothelial tumours: Enrichment and in vitro culture

Popis výsledku v původním jazyce

INTRODUCTION: Results of clinical trials have demonstrated that circulating tumour cells (CTCs) are frequently detected in patients with urothelial tumours. The monitoring of CTCs has the potential to improve therapeutic management at an early stage andalso to identify patients with increased risk of tumour progression or recurrence before the onset of clinically detected metastasis. In this study, we report a new effectively simplified methodology for a separation and in vitro culturing of viable CTCsfrom peripheral blood. METHOD: We include patients diagnosed with 3 types of urothelial tumours (prostate cancer, urinary bladder cancer, and kidney cancer). A size-based separation method for viable CTC - enrichment from unclothed peripheral blood hasbeen introduced (MetaCell, Ostrava, Czech Republic). The enriched CTCs fraction was cultured directly on the separation membrane, or transferred from the membrane and cultured on any plastic surface or a microscopic slide. RESULTS: We rep

Název v anglickém jazyce

Circulating tumour cells in patients with urothelial tumours: Enrichment and in vitro culture

Popis výsledku anglicky

INTRODUCTION: Results of clinical trials have demonstrated that circulating tumour cells (CTCs) are frequently detected in patients with urothelial tumours. The monitoring of CTCs has the potential to improve therapeutic management at an early stage andalso to identify patients with increased risk of tumour progression or recurrence before the onset of clinically detected metastasis. In this study, we report a new effectively simplified methodology for a separation and in vitro culturing of viable CTCsfrom peripheral blood. METHOD: We include patients diagnosed with 3 types of urothelial tumours (prostate cancer, urinary bladder cancer, and kidney cancer). A size-based separation method for viable CTC - enrichment from unclothed peripheral blood hasbeen introduced (MetaCell, Ostrava, Czech Republic). The enriched CTCs fraction was cultured directly on the separation membrane, or transferred from the membrane and cultured on any plastic surface or a microscopic slide. RESULTS: We rep

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

FD - Onkologie a hematologie

OECD FORD obor

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Projekt

—

Návaznosti

S - Specificky vyzkum na vysokych skolach

Rok uplatnění

2014

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Canadian Urological Association Journal

ISSN

1911-6470

e-ISSN

—

Svazek periodika

8

Číslo periodika v rámci svazku

9-10

Stát vydavatele periodika

CA - Kanada

Počet stran výsledku

6

Strana od-do

"E715"-"E720"

Kód UT WoS článku

000347710000008

EID výsledku v databázi Scopus

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