Frequency-tuned contactless conductivity detector for the electrophoretic separation of clinical samples in capillaries with very small internal dimensions

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11120%2F17%3A43912635" target="_blank" >RIV/00216208:11120/17:43912635 - isvavai.cz</a>

Výsledek na webu

<a href="http://dx.doi.org/10.1002/jssc.201601213" target="_blank" >http://dx.doi.org/10.1002/jssc.201601213</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1002/jssc.201601213" target="_blank" >10.1002/jssc.201601213</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Frequency-tuned contactless conductivity detector for the electrophoretic separation of clinical samples in capillaries with very small internal dimensions

Popis výsledku v původním jazyce

An axial design of a capacitively coupled contactless conductivity detector was tested in combination with fused silica capillaries with internal diameters of 10, 15 and 25 μm, which are used for high-efficiency electrophoretic separation. The transmission of the signal in the detection probe dependent on the specific conductivity of the solution in the capillary in the range 0-278 mS.m(-1) has a complex character and a minimum appears on the curve at very low conductivities. The position of the minimum of the calibration dependence gradually shifts with decreasing frequency of the exciting signal from 1.0 to 0.25 MHz towards lower specific conductivity values. The presence of a minimum on the calibration curves is a natural property of the axial design of contactless conductivity detector, demonstrated by solution of the equivalent electrical circuit of the detection probe, and is specifically caused by the use of shielding foil. The behavior of contactless conductivity detector in the vicinity of the minimum was documented for practical separations of amino acids in solutions of 3.2 M acetic acid with addition of 0-50% v/v methanol.

Název v anglickém jazyce

Frequency-tuned contactless conductivity detector for the electrophoretic separation of clinical samples in capillaries with very small internal dimensions

Popis výsledku anglicky

An axial design of a capacitively coupled contactless conductivity detector was tested in combination with fused silica capillaries with internal diameters of 10, 15 and 25 μm, which are used for high-efficiency electrophoretic separation. The transmission of the signal in the detection probe dependent on the specific conductivity of the solution in the capillary in the range 0-278 mS.m(-1) has a complex character and a minimum appears on the curve at very low conductivities. The position of the minimum of the calibration dependence gradually shifts with decreasing frequency of the exciting signal from 1.0 to 0.25 MHz towards lower specific conductivity values. The presence of a minimum on the calibration curves is a natural property of the axial design of contactless conductivity detector, demonstrated by solution of the equivalent electrical circuit of the detection probe, and is specifically caused by the use of shielding foil. The behavior of contactless conductivity detector in the vicinity of the minimum was documented for practical separations of amino acids in solutions of 3.2 M acetic acid with addition of 0-50% v/v methanol.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10406 - Analytical chemistry

Projekt

<a href="/cs/project/GA15-03139S" target="_blank" >GA15-03139S: Nové elektroforetické přístupy pro studium obezity a diabetu</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2017

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Journal of Separation Science

ISSN

1615-9306

e-ISSN

—

Svazek periodika

40

Číslo periodika v rámci svazku

4

Stát vydavatele periodika

DE - Spolková republika Německo

Počet stran výsledku

8

Strana od-do

940-947

Kód UT WoS článku

000397828000015

EID výsledku v databázi Scopus

2-s2.0-85013270025