Coaxial flow-gating interface for capillary electrophoresis

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11120%2F17%3A43913438" target="_blank" >RIV/00216208:11120/17:43913438 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00216208:11310/17:10363645

Výsledek na webu

<a href="http://dx.doi.org/10.1002/jssc.201700412" target="_blank" >http://dx.doi.org/10.1002/jssc.201700412</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1002/jssc.201700412" target="_blank" >10.1002/jssc.201700412</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Coaxial flow-gating interface for capillary electrophoresis

Popis výsledku v původním jazyce

A coaxial flow-gating interface is described in which the separation capillary passes through the sampling capillary. Continuous flow of the sample solution flowing out of the sampling capillary is directed away from the injection end of the separation capillary by counter-current flow of the gating solution. During the injection, the flow of the gating solution is interrupted, so that a plug of solution is formed at the inlet into the separation capillary, from which the sample is hydrodynamically injected. Flow-gating interfaces are originally designed for on-line connection of capillary electrophoresis with analytical flow-through methods. The basic properties of the described coaxial flow-gating interface were obtained in a simplified arrangement in which a syringe pump with sample solution has substituted analytical flow-through method. Under the optimized conditions, the properties of the tested interface were determined by separation of K+, Ba2+, Na+, Mg2+ and Li+ ions in aqueous solution at equimolar concentrations of 50 μM. The repeatability of the migration times and peak areas evaluated for K+, Ba2+ and Li+ ions and expressed as relative standard deviation did not exceed 1.4%. The interface was used to determine lithium in mineral water and taurine in an energy drink.

Název v anglickém jazyce

Coaxial flow-gating interface for capillary electrophoresis

Popis výsledku anglicky

A coaxial flow-gating interface is described in which the separation capillary passes through the sampling capillary. Continuous flow of the sample solution flowing out of the sampling capillary is directed away from the injection end of the separation capillary by counter-current flow of the gating solution. During the injection, the flow of the gating solution is interrupted, so that a plug of solution is formed at the inlet into the separation capillary, from which the sample is hydrodynamically injected. Flow-gating interfaces are originally designed for on-line connection of capillary electrophoresis with analytical flow-through methods. The basic properties of the described coaxial flow-gating interface were obtained in a simplified arrangement in which a syringe pump with sample solution has substituted analytical flow-through method. Under the optimized conditions, the properties of the tested interface were determined by separation of K+, Ba2+, Na+, Mg2+ and Li+ ions in aqueous solution at equimolar concentrations of 50 μM. The repeatability of the migration times and peak areas evaluated for K+, Ba2+ and Li+ ions and expressed as relative standard deviation did not exceed 1.4%. The interface was used to determine lithium in mineral water and taurine in an energy drink.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10406 - Analytical chemistry

Projekt

<a href="/cs/project/GA15-03139S" target="_blank" >GA15-03139S: Nové elektroforetické přístupy pro studium obezity a diabetu</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2017

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Journal of Separation Science

ISSN

1615-9306

e-ISSN

—

Svazek periodika

40

Číslo periodika v rámci svazku

15

Stát vydavatele periodika

DE - Spolková republika Německo

Počet stran výsledku

6

Strana od-do

3138-3143

Kód UT WoS článku

000407039600012

EID výsledku v databázi Scopus

2-s2.0-85021275312