An air-assisted flow-gating interface for capillary electrophoresis

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11120%2F19%3A43917361" target="_blank" >RIV/00216208:11120/19:43917361 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00216208:11310/19:10385492

Výsledek na webu

<a href="https://doi.org/10.1002/elps.201800421" target="_blank" >https://doi.org/10.1002/elps.201800421</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1002/elps.201800421" target="_blank" >10.1002/elps.201800421</a>

Jazyk výsledku

angličtina

Název v původním jazyce

An air-assisted flow-gating interface for capillary electrophoresis

Popis výsledku v původním jazyce

A new kind of flow gating interface (FGI) has been designed for on-line connection of CE with flow-through analytical techniques. The sample is injected into the separation capillary from a space from which the BGE was forced out by compressed air. A drop of sample solution with a volume of 75 nL is formed between the outlet of the delivery capillary supplying the solution from the flow-through apparatus and the entrance to the CE capillary; the sample is hydrodynamically injected into the CE capillary from this drop. The sample is not mixed with the surrounding BGE solution during injection. The functioning of the proposed FGI is fully automated and the individual steps of the injection process are controlled by a computer. The injection sequence lasts several seconds and thus permits performance of rapid sequential analyses of the collected sample. FGI was tested for the separation of equimolar 50 μM mixture of the inorganic cations K + , Ba 2+ , Na + , Mg 2+ and Li + in 50 mM acetic acid/20 mM Tris (pH 4.5) as BGE. The obtained RSD values for the migration times varied in the range 0.7-1.0% and the values for the peak area were 0.7-1.4%; RSD were determined for 10 repeated measurements.

Název v anglickém jazyce

An air-assisted flow-gating interface for capillary electrophoresis

Popis výsledku anglicky

A new kind of flow gating interface (FGI) has been designed for on-line connection of CE with flow-through analytical techniques. The sample is injected into the separation capillary from a space from which the BGE was forced out by compressed air. A drop of sample solution with a volume of 75 nL is formed between the outlet of the delivery capillary supplying the solution from the flow-through apparatus and the entrance to the CE capillary; the sample is hydrodynamically injected into the CE capillary from this drop. The sample is not mixed with the surrounding BGE solution during injection. The functioning of the proposed FGI is fully automated and the individual steps of the injection process are controlled by a computer. The injection sequence lasts several seconds and thus permits performance of rapid sequential analyses of the collected sample. FGI was tested for the separation of equimolar 50 μM mixture of the inorganic cations K + , Ba 2+ , Na + , Mg 2+ and Li + in 50 mM acetic acid/20 mM Tris (pH 4.5) as BGE. The obtained RSD values for the migration times varied in the range 0.7-1.0% and the values for the peak area were 0.7-1.4%; RSD were determined for 10 repeated measurements.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10406 - Analytical chemistry

Projekt

<a href="/cs/project/GA18-04902S" target="_blank" >GA18-04902S: Instrumentace pro kontinuální on-line elektroforetické monitorování metabolických procesů v živých organismech</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2019

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Electrophoresis

ISSN

0173-0835

e-ISSN

—

Svazek periodika

40

Číslo periodika v rámci svazku

4

Stát vydavatele periodika

DE - Spolková republika Německo

Počet stran výsledku

4

Strana od-do

587-590

Kód UT WoS článku

000458816900014

EID výsledku v databázi Scopus

2-s2.0-85057066156