Monitoring of adipose tissue metabolism using microdialysis and capillary electrophoresis with contactless conductivity detection

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11120%2F19%3A43917158" target="_blank" >RIV/00216208:11120/19:43917158 - isvavai.cz</a>

Výsledek na webu

<a href="https://doi.org/10.1016/j.talanta.2018.09.076" target="_blank" >https://doi.org/10.1016/j.talanta.2018.09.076</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.talanta.2018.09.076" target="_blank" >10.1016/j.talanta.2018.09.076</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Monitoring of adipose tissue metabolism using microdialysis and capillary electrophoresis with contactless conductivity detection

Popis výsledku v původním jazyce

Metabolism of human adipose tissue during acute exercise is monitored by microdialysis. The metabolites are analysed in the microdialysate using capillary electrophoresis (CE) on a short separation path in combination with a contactless conductivity detector (C4D). Four completely new CE/C4D methods were developed for determination of nutrients and metabolites in 10 µL of microdialysate. All methods are characterised by a short separation time and simple sample preparation based primarily on 4-fold dilution of microdialysate. The intra-day repeatability for the migration time varied in the range 0.4 - 0.9% and that for the peak area equalled 0.7 - 2.4%; the inter-day repeatability of the migration time was in the range 1.2 - 2.3% and the range for the peak area was 2.5 - 5.0%; all the values were measured as RSD. The developed determination was employed for sequenced monitoring of the levels of lactate, glycerol and branched chain amino acids in microdialysates taken from the abdominal adipose tissue during acute physical exercise. The stress test lasted 3 h and the metabolites were monitored at 15 min intervals.

Název v anglickém jazyce

Monitoring of adipose tissue metabolism using microdialysis and capillary electrophoresis with contactless conductivity detection

Popis výsledku anglicky

Metabolism of human adipose tissue during acute exercise is monitored by microdialysis. The metabolites are analysed in the microdialysate using capillary electrophoresis (CE) on a short separation path in combination with a contactless conductivity detector (C4D). Four completely new CE/C4D methods were developed for determination of nutrients and metabolites in 10 µL of microdialysate. All methods are characterised by a short separation time and simple sample preparation based primarily on 4-fold dilution of microdialysate. The intra-day repeatability for the migration time varied in the range 0.4 - 0.9% and that for the peak area equalled 0.7 - 2.4%; the inter-day repeatability of the migration time was in the range 1.2 - 2.3% and the range for the peak area was 2.5 - 5.0%; all the values were measured as RSD. The developed determination was employed for sequenced monitoring of the levels of lactate, glycerol and branched chain amino acids in microdialysates taken from the abdominal adipose tissue during acute physical exercise. The stress test lasted 3 h and the metabolites were monitored at 15 min intervals.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10406 - Analytical chemistry

Projekt

<a href="/cs/project/GA18-04902S" target="_blank" >GA18-04902S: Instrumentace pro kontinuální on-line elektroforetické monitorování metabolických procesů v živých organismech</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2019

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Talanta

ISSN

0039-9140

e-ISSN

—

Svazek periodika

192

Číslo periodika v rámci svazku

January

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

7

Strana od-do

380-386

Kód UT WoS článku

000449443900053

EID výsledku v databázi Scopus

2-s2.0-85053837405