A Validatation of non-invasive method for diagnostic of cystic fibrosis based on ion ratios determined by CE-C4D

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68081715%3A_____%2F18%3A00498535" target="_blank" >RIV/68081715:_____/18:00498535 - isvavai.cz</a>

Výsledek na webu

<a href="http://www.ce-ce.org/user_uploads/program/CECE%202018%20Proceedings_WOS.pdf" target="_blank" >http://www.ce-ce.org/user_uploads/program/CECE%202018%20Proceedings_WOS.pdf</a>

DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

A Validatation of non-invasive method for diagnostic of cystic fibrosis based on ion ratios determined by CE-C4D

Popis výsledku v původním jazyce

A validation of novel approach for diagnosis of cystic fibrosis (CF) is presented. The method is very simple and fast, since for collection of samples, only wiping of the skin of forearm is needed. The collected sample is analyzed by capillary electrophoresis with contactless conductometric detection (CE-C4D). Ion ratios are determined as ratios of peak area of chloride anion and peak areas of selected cations. It is possible to distinguish between cystic fibrosis patients and healthy individuals when ion ratio is applied as diagnostic factor. In here, we present analytical parameters of developed method (limits of detection, limits of quantification, repeatability of migration times, repeatability of peak areas) and ROC curve, which reveals high sensitivity and specificity of the developed method.

Název v anglickém jazyce

A Validatation of non-invasive method for diagnostic of cystic fibrosis based on ion ratios determined by CE-C4D

Popis výsledku anglicky

A validation of novel approach for diagnosis of cystic fibrosis (CF) is presented. The method is very simple and fast, since for collection of samples, only wiping of the skin of forearm is needed. The collected sample is analyzed by capillary electrophoresis with contactless conductometric detection (CE-C4D). Ion ratios are determined as ratios of peak area of chloride anion and peak areas of selected cations. It is possible to distinguish between cystic fibrosis patients and healthy individuals when ion ratio is applied as diagnostic factor. In here, we present analytical parameters of developed method (limits of detection, limits of quantification, repeatability of migration times, repeatability of peak areas) and ROC curve, which reveals high sensitivity and specificity of the developed method.

Druh

D - Stať ve sborníku

CEP obor

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OECD FORD obor

10406 - Analytical chemistry

Projekt

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Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2018

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název statě ve sborníku

CECE 2018. 15th International Interdisciplinary Meeting on Bioanalysis

ISBN

978-80-904959-5-1

ISSN

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e-ISSN

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Počet stran výsledku

6

Strana od-do

126-131

Název nakladatele

Ústav analytické chemie AV ČR, v. v. i.

Místo vydání

Brno

Místo konání akce

Brno

Datum konání akce

15. 10. 2018

Typ akce podle státní příslušnosti

WRD - Celosvětová akce

Kód UT WoS článku

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