NON-INVASIVE SAMPLING AND SUBSEQUENT CAPILLARY ELECTROPHORETIC ANALYSIS OF SWEAT AND SALIVA IN CLINICAL DIAGNOSIS OF CYSTIC FIBROSIS
Identifikátory výsledku
Kód výsledku v IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F14%3A00083393" target="_blank" >RIV/00216224:14310/14:00083393 - isvavai.cz</a>
Výsledek na webu
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DOI - Digital Object Identifier
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Alternativní jazyky
Jazyk výsledku
angličtina
Název v původním jazyce
NON-INVASIVE SAMPLING AND SUBSEQUENT CAPILLARY ELECTROPHORETIC ANALYSIS OF SWEAT AND SALIVA IN CLINICAL DIAGNOSIS OF CYSTIC FIBROSIS
Popis výsledku v původním jazyce
Capillary electrophoresis (CE) with double opposite end injection (DOEI) and contactless conductivity detection (C4D) was used in a novel approach for diagnosis of cystic fibrosis (CF). A simple, fast and inexpensive "skin wipe" technique for sweat sampling was developed as a time-saving substitute for conventional, iontophoretic sweat sampling. Saliva sample was also obtained. A number of major target analytes (inorganic/organic anions and inorganic cations) were quantified simultaneously in these samples. By applying principal component analysis (PCA) to ion concentration ratios, rather than to individual ion concentrations, more accurate diagnostic tool for CF was obtained. We demonstrate that by using the developed approach and comparing a group ofhealthy individuals to patients diagnosed with CF, two clearly distinguished clusters can be observed without any data overlap. This approach may thus set a basis for a new method to diagnose CF more reliably.
Název v anglickém jazyce
NON-INVASIVE SAMPLING AND SUBSEQUENT CAPILLARY ELECTROPHORETIC ANALYSIS OF SWEAT AND SALIVA IN CLINICAL DIAGNOSIS OF CYSTIC FIBROSIS
Popis výsledku anglicky
Capillary electrophoresis (CE) with double opposite end injection (DOEI) and contactless conductivity detection (C4D) was used in a novel approach for diagnosis of cystic fibrosis (CF). A simple, fast and inexpensive "skin wipe" technique for sweat sampling was developed as a time-saving substitute for conventional, iontophoretic sweat sampling. Saliva sample was also obtained. A number of major target analytes (inorganic/organic anions and inorganic cations) were quantified simultaneously in these samples. By applying principal component analysis (PCA) to ion concentration ratios, rather than to individual ion concentrations, more accurate diagnostic tool for CF was obtained. We demonstrate that by using the developed approach and comparing a group ofhealthy individuals to patients diagnosed with CF, two clearly distinguished clusters can be observed without any data overlap. This approach may thus set a basis for a new method to diagnose CF more reliably.
Klasifikace
Druh
D - Stať ve sborníku
CEP obor
CB - Analytická chemie, separace
OECD FORD obor
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Návaznosti výsledku
Projekt
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Návaznosti
S - Specificky vyzkum na vysokych skolach
Ostatní
Rok uplatnění
2014
Kód důvěrnosti údajů
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Údaje specifické pro druh výsledku
Název statě ve sborníku
CECE 2014: 11TH INTERNATIONAL INTERDISCIPLINARY MEETING ON BIOANALYSIS
ISBN
9788090495920
ISSN
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e-ISSN
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Počet stran výsledku
5
Strana od-do
182-186
Název nakladatele
INST ANALYTICAL CHEMISTRY ASCR, V V I-IAC
Místo vydání
BRNO
Místo konání akce
Brno, CZECH REPUBLIC
Datum konání akce
20. 10. 2014
Typ akce podle státní příslušnosti
EUR - Evropská akce
Kód UT WoS článku
000354547400061