Stearate-induced apoptosis in human pancreatic β-cells is associated with changes in membrane protein expression and these changes are inhibited by oleate

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11120%2F19%3A43917589" target="_blank" >RIV/00216208:11120/19:43917589 - isvavai.cz</a>

Výsledek na webu

<a href="https://doi.org/10.1002/prca.201800104" target="_blank" >https://doi.org/10.1002/prca.201800104</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1002/prca.201800104" target="_blank" >10.1002/prca.201800104</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Stearate-induced apoptosis in human pancreatic β-cells is associated with changes in membrane protein expression and these changes are inhibited by oleate

Popis výsledku v původním jazyce

PURPOSE: Lipotoxicity is implicated in type 2 diabetes pathogenesis. Its molecular mechanisms are not completely understood. The aim of this study is to identify new suspect proteins involved in pancreatic β-cell death induction by saturated fatty acids and its inhibition by unsaturated fatty acids. EXPERIMENTAL DESIGN: Employing 2DE analysis and subsequent western blot confirmation, we assessed the differences in membrane/membrane-associated protein expression in human β-cell line NES2Y during cell death induction by stearate and its inhibition by oleate. RESULTS: Induction of apoptosis by stearate was associated with significantly increased levels of Hsp90β, peroxiredoxin-1, and 14-3-3γ in the membrane fraction of NES2Y cells and significantly decreased levels of annexin A2, annexin A4, and reticulocalbin-2. All these changes were significantly inhibited by oleate co-application. No expression changes were detected after application of stearate together with oleate. Furthermore, the expression of reticulocalbin-2 was significantly decreased after stearate application also in the whole cell lysate. CONCLUSIONS AND CLINICAL RELEVANCE: We identified several membrane-associated proteins that could be related to pro- and anti-apoptotic signaling initiated by fatty acids in human pancreatic β-cells. As far as we know, annexin A4, reticulocalbin-2, and 14-3-3γ represent novel molecules related to the effect of fatty acids on β-cell viability.

Název v anglickém jazyce

Stearate-induced apoptosis in human pancreatic β-cells is associated with changes in membrane protein expression and these changes are inhibited by oleate

Popis výsledku anglicky

PURPOSE: Lipotoxicity is implicated in type 2 diabetes pathogenesis. Its molecular mechanisms are not completely understood. The aim of this study is to identify new suspect proteins involved in pancreatic β-cell death induction by saturated fatty acids and its inhibition by unsaturated fatty acids. EXPERIMENTAL DESIGN: Employing 2DE analysis and subsequent western blot confirmation, we assessed the differences in membrane/membrane-associated protein expression in human β-cell line NES2Y during cell death induction by stearate and its inhibition by oleate. RESULTS: Induction of apoptosis by stearate was associated with significantly increased levels of Hsp90β, peroxiredoxin-1, and 14-3-3γ in the membrane fraction of NES2Y cells and significantly decreased levels of annexin A2, annexin A4, and reticulocalbin-2. All these changes were significantly inhibited by oleate co-application. No expression changes were detected after application of stearate together with oleate. Furthermore, the expression of reticulocalbin-2 was significantly decreased after stearate application also in the whole cell lysate. CONCLUSIONS AND CLINICAL RELEVANCE: We identified several membrane-associated proteins that could be related to pro- and anti-apoptotic signaling initiated by fatty acids in human pancreatic β-cells. As far as we know, annexin A4, reticulocalbin-2, and 14-3-3γ represent novel molecules related to the effect of fatty acids on β-cell viability.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

30202 - Endocrinology and metabolism (including diabetes, hormones)

Projekt

<a href="/cs/project/GP14-00630P" target="_blank" >GP14-00630P: Regulace indukce apoptózy nasycenými a nenasycenými mastnými kyselinami u pankreatických beta buněk: protemický přístup</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2019

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Proteomics: Clinical Applications

ISSN

1862-8346

e-ISSN

—

Svazek periodika

13

Číslo periodika v rámci svazku

4

Stát vydavatele periodika

DE - Spolková republika Německo

Počet stran výsledku

10

Strana od-do

"Article 1800104"

Kód UT WoS článku

000475990600020

EID výsledku v databázi Scopus

2-s2.0-85060917976