Stearate-Induced Apoptosis in Human Pancreatic beta-Cells is Associated with Changes in Membrane Protein Expression and These Changes are Inhibited by Oleate

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388971%3A_____%2F19%3A00507955" target="_blank" >RIV/61388971:_____/19:00507955 - isvavai.cz</a>

Výsledek na webu

<a href="https://onlinelibrary.wiley.com/doi/abs/10.1002/prca.201800104" target="_blank" >https://onlinelibrary.wiley.com/doi/abs/10.1002/prca.201800104</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1002/prca.201800104" target="_blank" >10.1002/prca.201800104</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Stearate-Induced Apoptosis in Human Pancreatic beta-Cells is Associated with Changes in Membrane Protein Expression and These Changes are Inhibited by Oleate

Popis výsledku v původním jazyce

Purpose Lipotoxicity is implicated in type 2 diabetes pathogenesis. Its molecular mechanisms are not completely understood. The aim of this study is to identify new suspect proteins involved in pancreatic beta-cell death induction by saturated fatty acids and its inhibition by unsaturated fatty acids. Experimental design Employing 2DE analysis and subsequent western blot confirmation, the differences in membrane/membrane-associated protein expression in human beta-cell line NES2Y are assessed during cell death induction by stearate and its inhibition by oleate. Results Induction of apoptosis by stearate is associated with significantly increased levels of Hsp90 beta, peroxiredoxin-1, and 14-3-3 gamma in the membrane fraction of NES2Y cells and significantly decreased levels of annexin A2, annexin A4, and reticulocalbin-2. All these changes are significantly inhibited by oleate co-application. No expression changes are detected after application of stearate together with oleate. Furthermore, the expression of reticulocalbin-2 is significantly decreased after stearate application also in the whole cell lysate. Conclusions and clinical relevance Several membrane-associated proteins that could be related to pro- and anti-apoptotic signaling initiated by fatty acids in human pancreatic beta-cells are identified. As far as we know, annexin A4, reticulocalbin-2, and 14-3-3 gamma represent novel molecules related to the effect of fatty acids on beta-cell viability.

Název v anglickém jazyce

Stearate-Induced Apoptosis in Human Pancreatic beta-Cells is Associated with Changes in Membrane Protein Expression and These Changes are Inhibited by Oleate

Popis výsledku anglicky

Purpose Lipotoxicity is implicated in type 2 diabetes pathogenesis. Its molecular mechanisms are not completely understood. The aim of this study is to identify new suspect proteins involved in pancreatic beta-cell death induction by saturated fatty acids and its inhibition by unsaturated fatty acids. Experimental design Employing 2DE analysis and subsequent western blot confirmation, the differences in membrane/membrane-associated protein expression in human beta-cell line NES2Y are assessed during cell death induction by stearate and its inhibition by oleate. Results Induction of apoptosis by stearate is associated with significantly increased levels of Hsp90 beta, peroxiredoxin-1, and 14-3-3 gamma in the membrane fraction of NES2Y cells and significantly decreased levels of annexin A2, annexin A4, and reticulocalbin-2. All these changes are significantly inhibited by oleate co-application. No expression changes are detected after application of stearate together with oleate. Furthermore, the expression of reticulocalbin-2 is significantly decreased after stearate application also in the whole cell lysate. Conclusions and clinical relevance Several membrane-associated proteins that could be related to pro- and anti-apoptotic signaling initiated by fatty acids in human pancreatic beta-cells are identified. As far as we know, annexin A4, reticulocalbin-2, and 14-3-3 gamma represent novel molecules related to the effect of fatty acids on beta-cell viability.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10608 - Biochemistry and molecular biology

Projekt

—

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2019

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Proteomics Clinical Applications

ISSN

1862-8346

e-ISSN

—

Svazek periodika

13

Číslo periodika v rámci svazku

4

Stát vydavatele periodika

DE - Spolková republika Německo

Počet stran výsledku

10

Strana od-do

1800104

Kód UT WoS článku

000475990600020

EID výsledku v databázi Scopus

2-s2.0-85060917976