Whole genome sequencing of macrolide resistant Streptococcus pneumoniae serotype 19A sequence type 416
Identifikátory výsledku
Kód výsledku v IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11120%2F20%3A43920315" target="_blank" >RIV/00216208:11120/20:43920315 - isvavai.cz</a>
Nalezeny alternativní kódy
RIV/00216208:11150/20:10412967 RIV/00179906:_____/20:10412967 RIV/75010330:_____/20:00013024 RIV/00216208:11140/20:10412967 RIV/00064173:_____/20:N0000200
Výsledek na webu
<a href="https://doi.org/10.1186/s12866-020-01909-1" target="_blank" >https://doi.org/10.1186/s12866-020-01909-1</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1186/s12866-020-01909-1" target="_blank" >10.1186/s12866-020-01909-1</a>
Alternativní jazyky
Jazyk výsledku
angličtina
Název v původním jazyce
Whole genome sequencing of macrolide resistant Streptococcus pneumoniae serotype 19A sequence type 416
Popis výsledku v původním jazyce
BACKGROUND: The resistance of Streptococcus pneumoniae to macrolides is becoming an increasingly important issue and thus it is important to understand the genetics related to adaptation of this species to the widespread use of antibiotics in Europe. The 58 isolates of S. pneumoniae belonging to sequence type (ST) 416 and serotype 19A and to several different phenotypes originated from Italy, Portugal and Czech Republic were thus sequenced on Illumina MiSeq. The aim of the study was to describe genetical origine of isolates, investigate their macrolide resistance and suggest reasons for spread of ST416 in the Czech Republic. RESULTS: Investigation of genes associated with serotype determined serotype switch between 15B and 19A serotypes and core genome multilocus sequence typing (cgMLST) confirmed the origine of concerned isolates in Netherlands-37 clone. Inspected genomes proved variability of genes associated with the macrolide resistance even within closely genetically relative isolates. CONCLUSIONS: Participation of 19A/ST416 on the spread of Netherlands-37 is accompanied by serotype switch between 19A and 15B serotypes and with acquisition of genes involved in macrolide resistance to the clone that was originally macrolide susceptible. There is evident tendency to interchanging and modifications of these and surrounding genes, that could lead to accelerate spreading of this sequence type in regions with high macrolide consumption.
Název v anglickém jazyce
Whole genome sequencing of macrolide resistant Streptococcus pneumoniae serotype 19A sequence type 416
Popis výsledku anglicky
BACKGROUND: The resistance of Streptococcus pneumoniae to macrolides is becoming an increasingly important issue and thus it is important to understand the genetics related to adaptation of this species to the widespread use of antibiotics in Europe. The 58 isolates of S. pneumoniae belonging to sequence type (ST) 416 and serotype 19A and to several different phenotypes originated from Italy, Portugal and Czech Republic were thus sequenced on Illumina MiSeq. The aim of the study was to describe genetical origine of isolates, investigate their macrolide resistance and suggest reasons for spread of ST416 in the Czech Republic. RESULTS: Investigation of genes associated with serotype determined serotype switch between 15B and 19A serotypes and core genome multilocus sequence typing (cgMLST) confirmed the origine of concerned isolates in Netherlands-37 clone. Inspected genomes proved variability of genes associated with the macrolide resistance even within closely genetically relative isolates. CONCLUSIONS: Participation of 19A/ST416 on the spread of Netherlands-37 is accompanied by serotype switch between 19A and 15B serotypes and with acquisition of genes involved in macrolide resistance to the clone that was originally macrolide susceptible. There is evident tendency to interchanging and modifications of these and surrounding genes, that could lead to accelerate spreading of this sequence type in regions with high macrolide consumption.
Klasifikace
Druh
J<sub>imp</sub> - Článek v periodiku v databázi Web of Science
CEP obor
—
OECD FORD obor
10606 - Microbiology
Návaznosti výsledku
Projekt
<a href="/cs/project/NV16-27109A" target="_blank" >NV16-27109A: Vývoj multirezistentního Streptococcus pneumoniae sérotypu 19A v éře vakcinace proti pneumokokům</a><br>
Návaznosti
V - Vyzkumna aktivita podporovana z jinych verejnych zdroju
Ostatní
Rok uplatnění
2020
Kód důvěrnosti údajů
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Údaje specifické pro druh výsledku
Název periodika
BMC Microbiology
ISSN
1471-2180
e-ISSN
—
Svazek periodika
20
Číslo periodika v rámci svazku
July
Stát vydavatele periodika
GB - Spojené království Velké Británie a Severního Irska
Počet stran výsledku
10
Strana od-do
"Article 224"
Kód UT WoS článku
000555944000002
EID výsledku v databázi Scopus
2-s2.0-85088622988