A new electromembrane extraction probe for on-line connection with capillary electrophoresis for determination of substances in biological matrices

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11120%2F23%3A43924371" target="_blank" >RIV/00216208:11120/23:43924371 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00216208:11310/23:10451191

Výsledek na webu

<a href="https://doi.org/10.1016/j.talanta.2022.124149" target="_blank" >https://doi.org/10.1016/j.talanta.2022.124149</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.talanta.2022.124149" target="_blank" >10.1016/j.talanta.2022.124149</a>

Jazyk výsledku

angličtina

Název v původním jazyce

A new electromembrane extraction probe for on-line connection with capillary electrophoresis for determination of substances in biological matrices

Popis výsledku v původním jazyce

A miniature probe for electromembrane extraction is developed and constructed. The tubular probe with an internal volume of 1.1 μL is made of polypropylene hollow fiber with a supported liquid membrane of 85% nitrophenyloctyl ether (NPOE) with 15% bis(2-ethylhexyl)phosphonic acid (DEHP). The probe is connected on-line to the electrophoresis with short separation capillary via an air assisted flow gating interface cast from poly (dimethylsiloxane). The compact instrument is computer controlled via LabView. The probe parameters are tested for extraction of creatinine and basic amino acids from artificial solution and human urine. The sensitivity of the electrophoretic determination after 300 s extraction at 150 V compared to the sensitivity without extraction is 4.9-fold and 2.6-fold higher for creatinine and arginine, respectively. The RSDs for peak area measured from 5 repeated extractions of 50 μM solutions are 7.5%, 7.2%, 8.6% and 9.2% for Crea, Lys, Arg and His, respectively. The probe can be used for all-day measurements. The preparation of the probe is simple and requires no special tool.

Název v anglickém jazyce

A new electromembrane extraction probe for on-line connection with capillary electrophoresis for determination of substances in biological matrices

Popis výsledku anglicky

A miniature probe for electromembrane extraction is developed and constructed. The tubular probe with an internal volume of 1.1 μL is made of polypropylene hollow fiber with a supported liquid membrane of 85% nitrophenyloctyl ether (NPOE) with 15% bis(2-ethylhexyl)phosphonic acid (DEHP). The probe is connected on-line to the electrophoresis with short separation capillary via an air assisted flow gating interface cast from poly (dimethylsiloxane). The compact instrument is computer controlled via LabView. The probe parameters are tested for extraction of creatinine and basic amino acids from artificial solution and human urine. The sensitivity of the electrophoretic determination after 300 s extraction at 150 V compared to the sensitivity without extraction is 4.9-fold and 2.6-fold higher for creatinine and arginine, respectively. The RSDs for peak area measured from 5 repeated extractions of 50 μM solutions are 7.5%, 7.2%, 8.6% and 9.2% for Crea, Lys, Arg and His, respectively. The probe can be used for all-day measurements. The preparation of the probe is simple and requires no special tool.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10406 - Analytical chemistry

Projekt

<a href="/cs/project/GA22-22398S" target="_blank" >GA22-22398S: Mikrofluidní a elektronická zařízení pro on-line elektroforetickou analýzu tukové tkáně</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2023

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Talanta

ISSN

0039-9140

e-ISSN

1873-3573

Svazek periodika

254

Číslo periodika v rámci svazku

March

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

5

Strana od-do

124149

Kód UT WoS článku

001003492200003

EID výsledku v databázi Scopus

2-s2.0-85143527215