Region-specific modulation of limbic seizure susceptibility by ovarian steroids

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11120%2F99%3A43903511" target="_blank" >RIV/00216208:11120/99:43903511 - isvavai.cz</a>

Výsledek na webu

<a href="http://dx.doi.org/10.1016/S0006-8993(99)01858-2" target="_blank" >http://dx.doi.org/10.1016/S0006-8993(99)01858-2</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/S0006-8993(99)01858-2" target="_blank" >10.1016/S0006-8993(99)01858-2</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Region-specific modulation of limbic seizure susceptibility by ovarian steroids

Popis výsledku v původním jazyce

Gonadal steroid hormones can markedly affect seizure susceptibility. Ovariohysterectomized female rats given ovarian steroid hormone supplements were used to evaluate the effects of ovarian steroids on epileptiform activity in hippocampal slices in vitro and on flurothyl-induced seizures in vivo. Seizure susceptibility was compared in the entorhinal cortex (EC) and CA1 regions of the hippocampus perfused with Mg2+-free medium, which leads to epileptiform discharges caused by a relief of voltage-dependent NMDA receptor block. After in vivo treatment with 500 mu g of progesterone for 2 h prior to slice preparation, the latency to onset of low Mg2+-induced epileptiform activity of slices was significantly prolonged compared to slices from controls. In contrast, progesterone replacement accelerated the development of epileptiform activity in the CAL region, Neither estrogen alone (2 x 2 mu g of estradiol benzoate, 48 and 24 h prior to the experiment), nor a combined treatment with estrogen plus progesterone, significantly affected seizure susceptibility in either CAI or the EC. There were no consistent effects of estrogen or progesterone, alone or in combination, on flurothyl-induced seizures in vivo. The data suggest that in vitro, progesterone alters seizure susceptibility in a site and seizure model-specific fashion. The differential effects of progesterone may be due to differential expression of progesterone receptor isoforms or metabolites in specific brain areas suggesting that selective modulation of NMDA receptor-dependent epileptiform activity may play a role in hormonal effects on epileptogenesis.

Název v anglickém jazyce

Region-specific modulation of limbic seizure susceptibility by ovarian steroids

Popis výsledku anglicky

Gonadal steroid hormones can markedly affect seizure susceptibility. Ovariohysterectomized female rats given ovarian steroid hormone supplements were used to evaluate the effects of ovarian steroids on epileptiform activity in hippocampal slices in vitro and on flurothyl-induced seizures in vivo. Seizure susceptibility was compared in the entorhinal cortex (EC) and CA1 regions of the hippocampus perfused with Mg2+-free medium, which leads to epileptiform discharges caused by a relief of voltage-dependent NMDA receptor block. After in vivo treatment with 500 mu g of progesterone for 2 h prior to slice preparation, the latency to onset of low Mg2+-induced epileptiform activity of slices was significantly prolonged compared to slices from controls. In contrast, progesterone replacement accelerated the development of epileptiform activity in the CAL region, Neither estrogen alone (2 x 2 mu g of estradiol benzoate, 48 and 24 h prior to the experiment), nor a combined treatment with estrogen plus progesterone, significantly affected seizure susceptibility in either CAI or the EC. There were no consistent effects of estrogen or progesterone, alone or in combination, on flurothyl-induced seizures in vivo. The data suggest that in vitro, progesterone alters seizure susceptibility in a site and seizure model-specific fashion. The differential effects of progesterone may be due to differential expression of progesterone receptor isoforms or metabolites in specific brain areas suggesting that selective modulation of NMDA receptor-dependent epileptiform activity may play a role in hormonal effects on epileptogenesis.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

ED - Fyziologie

OECD FORD obor

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Projekt

<a href="/cs/project/IZ3613" target="_blank" >IZ3613: Vliv chronické terapie antiepileptiky a časných postnatálních záchvatů na vývoj normálních mozkových funkcí</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

1999

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Brain Research

ISSN

0006-8993

e-ISSN

—

Svazek periodika

842

Číslo periodika v rámci svazku

1

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

7

Strana od-do

132-138

Kód UT WoS článku

000082809300015

EID výsledku v databázi Scopus

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