DNA damage, DNA repair rates and mRNA expression levels of cell cycle genes (Tp53, p21(CDKNIA), BCL2 and BAX) with respect to occupational exposure to styrene

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11130%2F11%3A6989" target="_blank" >RIV/00216208:11130/11:6989 - isvavai.cz</a>

Výsledek na webu

<a href="http://www.ncbi.nlm.nih.gov/pubmed/20966084" target="_blank" >http://www.ncbi.nlm.nih.gov/pubmed/20966084</a>

DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

DNA damage, DNA repair rates and mRNA expression levels of cell cycle genes (Tp53, p21(CDKNIA), BCL2 and BAX) with respect to occupational exposure to styrene

Popis výsledku v původním jazyce

We studied the relationship between DNA damage, DNA repair rates and messenger RNA (mRNA) expression levels of cell cycle genes TP53, p21(CDKNIA), BCL2 and BAX in a group of 71 styrene-exposed workers and 51 control individuals. The exposure was assessedby measuring the concentration of styrene at workplace and in blood. Parameters of DNA damage [measured as single-strand breaks (SSBs) and endonuclease III-sensitive sites], gamma-irradiation-specific DNA repair rates and mRNA levels of studied genes were analyzed in peripheral blood lymphocytes. The workers were divided into low (<50 mg/m(3)) and high (>50 mg/m(3)) styrene exposure groups. We found negative correlations between mRNA expression of TP53, BCL2, BAX and styrene exposure (P < 0.001 for allparameters). In contrast, p21(CDKNIA) mRNA expression significantly increased with increasing styrene exposure (P = 0.001). SSBs and endonuclease Insensitive sites increased with increasing mRNA levels of TP53 (P < 0.001 for both) and BC

Název v anglickém jazyce

DNA damage, DNA repair rates and mRNA expression levels of cell cycle genes (Tp53, p21(CDKNIA), BCL2 and BAX) with respect to occupational exposure to styrene

Popis výsledku anglicky

We studied the relationship between DNA damage, DNA repair rates and messenger RNA (mRNA) expression levels of cell cycle genes TP53, p21(CDKNIA), BCL2 and BAX in a group of 71 styrene-exposed workers and 51 control individuals. The exposure was assessedby measuring the concentration of styrene at workplace and in blood. Parameters of DNA damage [measured as single-strand breaks (SSBs) and endonuclease III-sensitive sites], gamma-irradiation-specific DNA repair rates and mRNA levels of studied genes were analyzed in peripheral blood lymphocytes. The workers were divided into low (<50 mg/m(3)) and high (>50 mg/m(3)) styrene exposure groups. We found negative correlations between mRNA expression of TP53, BCL2, BAX and styrene exposure (P < 0.001 for allparameters). In contrast, p21(CDKNIA) mRNA expression significantly increased with increasing styrene exposure (P = 0.001). SSBs and endonuclease Insensitive sites increased with increasing mRNA levels of TP53 (P < 0.001 for both) and BC

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

FD - Onkologie a hematologie

OECD FORD obor

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Projekt

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Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2011

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Carcinogenesis

ISSN

0143-3334

e-ISSN

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Svazek periodika

32

Číslo periodika v rámci svazku

1

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

6

Strana od-do

74-79

Kód UT WoS článku

000286303000012

EID výsledku v databázi Scopus

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