Altered Astrocytic Swelling in the Cortex of alpha-Syntrophin-Negative GFAP/EGFP Mice

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11130%2F14%3A10293164" target="_blank" >RIV/00216208:11130/14:10293164 - isvavai.cz</a>

Výsledek na webu

<a href="http://dx.doi.org/10.1371/journal.pone.0113444" target="_blank" >http://dx.doi.org/10.1371/journal.pone.0113444</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1371/journal.pone.0113444" target="_blank" >10.1371/journal.pone.0113444</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Altered Astrocytic Swelling in the Cortex of alpha-Syntrophin-Negative GFAP/EGFP Mice

Popis výsledku v původním jazyce

Brain edema accompanying ischemic or traumatic brain injuries, originates from a disruption of ionic/neurotransmitter homeostasis that leads to accumulation of K+ and glutamate in the extracellular space. Their increased uptake, predominantly provided byastrocytes, is associated with water influx via aquaporin-4 (AQP4). As the removal of perivascular AQP4 via the deletion of alpha-syntrophin was shown to delay edema formation and K+ clearance, we aimed to elucidate the impact of alpha-syntrophin knockout on volume changes in individual astrocytes in situ evoked by pathological stimuli using three dimensional confocal morphometry and changes in the extracellular space volume fraction (alpha) in situ and in vivo in the mouse cortex employing the real-time iontophoretic method. RT-qPCR profiling was used to reveal possible differences in the expression of ion channels/transporters that participate in maintaining ionic/neurotransmitter homeostasis. To visualize individual astrocytes in mi

Název v anglickém jazyce

Altered Astrocytic Swelling in the Cortex of alpha-Syntrophin-Negative GFAP/EGFP Mice

Popis výsledku anglicky

Brain edema accompanying ischemic or traumatic brain injuries, originates from a disruption of ionic/neurotransmitter homeostasis that leads to accumulation of K+ and glutamate in the extracellular space. Their increased uptake, predominantly provided byastrocytes, is associated with water influx via aquaporin-4 (AQP4). As the removal of perivascular AQP4 via the deletion of alpha-syntrophin was shown to delay edema formation and K+ clearance, we aimed to elucidate the impact of alpha-syntrophin knockout on volume changes in individual astrocytes in situ evoked by pathological stimuli using three dimensional confocal morphometry and changes in the extracellular space volume fraction (alpha) in situ and in vivo in the mouse cortex employing the real-time iontophoretic method. RT-qPCR profiling was used to reveal possible differences in the expression of ion channels/transporters that participate in maintaining ionic/neurotransmitter homeostasis. To visualize individual astrocytes in mi

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

FH - Neurologie, neurochirurgie, neurovědy

OECD FORD obor

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Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2014

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

PLoS ONE

ISSN

1932-6203

e-ISSN

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Svazek periodika

9

Číslo periodika v rámci svazku

11

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

34

Strana od-do

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Kód UT WoS článku

000349145400053

EID výsledku v databázi Scopus

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