Gut Virome Sequencing in Children With Early Islet Autoimmunity

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11130%2F15%3A10294477" target="_blank" >RIV/00216208:11130/15:10294477 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00064203:_____/15:10294477

Výsledek na webu

<a href="http://dx.doi.org/10.2337/dc14-2490" target="_blank" >http://dx.doi.org/10.2337/dc14-2490</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.2337/dc14-2490" target="_blank" >10.2337/dc14-2490</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Gut Virome Sequencing in Children With Early Islet Autoimmunity

Popis výsledku v původním jazyce

OBJECTIVEThis study used next-generation sequencing (NGS) technologies to characterize the gut virome at the onset of islet autoimmunity.RESEARCH DESIGN AND METHODSWe conducted a case-control study nested within the Finnish Diabetes Prediction and Prevention (DIPP) cohort. The stool virome in 19 case children, who turned islet autoantibody positive before the age of 2 years and later developed clinical type 1 diabetes, and 19 tightly matched control subjects was analyzed using NGS performed from stool samples collected 3, 6, and 9 months before the onset of islet autoimmunity. Human virus findings were verified using real-time PCR.RESULTSOne or more human viruses were present in 10.4% and bacteriophages were in 54% of the samples. The virome composition showed no association with islet autoimmunity. NGS was less sensitive and specific than real-time PCR.CONCLUSIONSThe present data suggest no dramatic changes in the gut virome shortly before the emergence of islet autoimmunity and empha

Název v anglickém jazyce

Gut Virome Sequencing in Children With Early Islet Autoimmunity

Popis výsledku anglicky

OBJECTIVEThis study used next-generation sequencing (NGS) technologies to characterize the gut virome at the onset of islet autoimmunity.RESEARCH DESIGN AND METHODSWe conducted a case-control study nested within the Finnish Diabetes Prediction and Prevention (DIPP) cohort. The stool virome in 19 case children, who turned islet autoantibody positive before the age of 2 years and later developed clinical type 1 diabetes, and 19 tightly matched control subjects was analyzed using NGS performed from stool samples collected 3, 6, and 9 months before the onset of islet autoimmunity. Human virus findings were verified using real-time PCR.RESULTSOne or more human viruses were present in 10.4% and bacteriophages were in 54% of the samples. The virome composition showed no association with islet autoimmunity. NGS was less sensitive and specific than real-time PCR.CONCLUSIONSThe present data suggest no dramatic changes in the gut virome shortly before the emergence of islet autoimmunity and empha

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

FG - Pediatrie

OECD FORD obor

—

Projekt

<a href="/cs/project/NT11465" target="_blank" >NT11465: Vybrané pikornaviry v patogenezi ostrůvkové autoimunity a diabetu 1. typu</a><br>

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2015

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Diabetes Care

ISSN

0149-5992

e-ISSN

—

Svazek periodika

38

Číslo periodika v rámci svazku

5

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

4

Strana od-do

930-933

Kód UT WoS článku

000353505600037

EID výsledku v databázi Scopus

2-s2.0-84939250362