Identification of bacteria from aerobic and anaerobic blood cultures after short cultivation by matrix-assisted laser desorption/ionization-time-of-flight mass spectrometry

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11130%2F15%3A10315938" target="_blank" >RIV/00216208:11130/15:10315938 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00064203:_____/15:10315938

Výsledek na webu

<a href="http://dx.doi.org/10.1099/jmm.0.000140" target="_blank" >http://dx.doi.org/10.1099/jmm.0.000140</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1099/jmm.0.000140" target="_blank" >10.1099/jmm.0.000140</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Identification of bacteria from aerobic and anaerobic blood cultures after short cultivation by matrix-assisted laser desorption/ionization-time-of-flight mass spectrometry

Popis výsledku v původním jazyce

Timely identification of bacterial agents that cause invasive bloodstream infections is of crucial importance. These clinical conditions require early and appropriate antibiotic intervention to prevent their progress into life-threatening septicaemia. Blood culture remains a gold standard technique for detection of bloodstream pathogens; however, its inherent time delay drives clinical microbiology laboratories to find methodological alternatives to speed up the whole process. PCR-based techniques are increasingly utilized for early diagnosis, but their application in detecting bacteria directly from blood is currently limited, mostly due to unresolved diagnostic accuracy and high costs. Recently, bacterial identification was accelerated tremendously by using matrix-assisted laser desorption/ionization-time-of-flight (MALDI-TOF) mass spectrometry. We aimed to develop an easy-to-implement MALDI-TOF-based method for identification of positive blood cultures that would substantially reduc

Název v anglickém jazyce

Identification of bacteria from aerobic and anaerobic blood cultures after short cultivation by matrix-assisted laser desorption/ionization-time-of-flight mass spectrometry

Popis výsledku anglicky

Timely identification of bacterial agents that cause invasive bloodstream infections is of crucial importance. These clinical conditions require early and appropriate antibiotic intervention to prevent their progress into life-threatening septicaemia. Blood culture remains a gold standard technique for detection of bloodstream pathogens; however, its inherent time delay drives clinical microbiology laboratories to find methodological alternatives to speed up the whole process. PCR-based techniques are increasingly utilized for early diagnosis, but their application in detecting bacteria directly from blood is currently limited, mostly due to unresolved diagnostic accuracy and high costs. Recently, bacterial identification was accelerated tremendously by using matrix-assisted laser desorption/ionization-time-of-flight (MALDI-TOF) mass spectrometry. We aimed to develop an easy-to-implement MALDI-TOF-based method for identification of positive blood cultures that would substantially reduc

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

EE - Mikrobiologie, virologie

OECD FORD obor

—

Projekt

—

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2015

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Journal of Medical Microbiology

ISSN

0022-2615

e-ISSN

—

Svazek periodika

64

Číslo periodika v rámci svazku

říjen

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

3

Strana od-do

1253-1255

Kód UT WoS článku

000365460900018

EID výsledku v databázi Scopus

2-s2.0-84944747078