The polymorphic insertion of the luteinizing hormone receptor "insLQ" show a negative association to LHR gene expression and to the follicular fluid hormonal profile in human small antral follicles

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11130%2F18%3A10375387" target="_blank" >RIV/00216208:11130/18:10375387 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00064203:_____/18:10375387

Výsledek na webu

<a href="https://doi.org/10.1016/j.mce.2017.07.002" target="_blank" >https://doi.org/10.1016/j.mce.2017.07.002</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.mce.2017.07.002" target="_blank" >10.1016/j.mce.2017.07.002</a>

Jazyk výsledku

angličtina

Název v původním jazyce

The polymorphic insertion of the luteinizing hormone receptor "insLQ" show a negative association to LHR gene expression and to the follicular fluid hormonal profile in human small antral follicles

Popis výsledku v původním jazyce

The luteinizing hormone receptor (LHCGR) has a little studied polymorphic 6 bp insertion (rs4539842/insLQ). This study has evaluated the insLQ polymorphism in relation to potential associations with hormonal characteristics of human small antral follicles (hSAFs). In total, 310 hSAFs were collected from 86 women undergoing fertility preservation. Analysis included hormonal profile of 297 follicular fluid (FF) samples and 148 corresponding granulosa cells samples were evaluated by cPCR for selected genes. Significantly reduced and non-detectable mRNA levels of anti-Mullerian hormone receptor II (AMHR2) and LHCGR, respectively, were observed for insLQ]insLQ compared to -/insLQ and the -/- genotypes. Moreover, LHCGR and CYP19a1 together with oestradiol and inhibin-B were significantly increased in -/insLQ compared to the -/- genotype. The homozygous insLQ genotype showed strong significant associations to GC specific genes LHCGR and CYP19a1, which may translate into significant changes in FF hormone profiles and an altered LH signaling.

Název v anglickém jazyce

The polymorphic insertion of the luteinizing hormone receptor "insLQ" show a negative association to LHR gene expression and to the follicular fluid hormonal profile in human small antral follicles

Popis výsledku anglicky

The luteinizing hormone receptor (LHCGR) has a little studied polymorphic 6 bp insertion (rs4539842/insLQ). This study has evaluated the insLQ polymorphism in relation to potential associations with hormonal characteristics of human small antral follicles (hSAFs). In total, 310 hSAFs were collected from 86 women undergoing fertility preservation. Analysis included hormonal profile of 297 follicular fluid (FF) samples and 148 corresponding granulosa cells samples were evaluated by cPCR for selected genes. Significantly reduced and non-detectable mRNA levels of anti-Mullerian hormone receptor II (AMHR2) and LHCGR, respectively, were observed for insLQ]insLQ compared to -/insLQ and the -/- genotypes. Moreover, LHCGR and CYP19a1 together with oestradiol and inhibin-B were significantly increased in -/insLQ compared to the -/- genotype. The homozygous insLQ genotype showed strong significant associations to GC specific genes LHCGR and CYP19a1, which may translate into significant changes in FF hormone profiles and an altered LH signaling.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10600 - Biological sciences

Projekt

—

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2018

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Molecular and Cellular Endocrinology

ISSN

0303-7207

e-ISSN

—

Svazek periodika

460

Číslo periodika v rámci svazku

15

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

6

Strana od-do

57-62

Kód UT WoS článku

000423893300006

EID výsledku v databázi Scopus

2-s2.0-85024474722