The bacteriome at the onset of type 1 diabetes: A study from four geographically distant African and Asian countries

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11130%2F18%3A10381686" target="_blank" >RIV/00216208:11130/18:10381686 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00064203:_____/18:10381686

Výsledek na webu

<a href="https://doi.org/10.1016/j.diabres.2018.08.010" target="_blank" >https://doi.org/10.1016/j.diabres.2018.08.010</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.diabres.2018.08.010" target="_blank" >10.1016/j.diabres.2018.08.010</a>

Jazyk výsledku

angličtina

Název v původním jazyce

The bacteriome at the onset of type 1 diabetes: A study from four geographically distant African and Asian countries

Popis výsledku v původním jazyce

Objectives: Gut bacteriome profiling studies in type 1 diabetes (T1D) to date are mostly limited to populations of Europe, with two studies from China and one study each from Mexico and the USA. We therefore sought to characterize the stool bacteriome in children after onset of T1D along with age-and place-matched control subjects from four geographically distant African and Asian countries. Methods: Samples were collected from 73 children and adolescents shortly after T1D onset (Azerbaijan 19, Jordan 20, Nigeria 14, Sudan 20) and 104 matched control subjects of similar age and locale. Genotyping of major T1D susceptibility genes was performed using saliva or blood samples. The bacteriome was profiled by next-generation sequencing of 16S rDNA. Negative binomial regression was used to model associations, with adjustment for the matched structure of the study. Results: A significant positive association with T1D was noted for the genus Escherichia (class Gammaproteobacteria, phylum Proteobacteria), whereas Eubacterium and Roseburia, two genera of class Clostridia, phylum Firmicutes, were inversely associated with T1D. We also confirmed a previously observed inverse association with Clostridium clusters IV or XIVa. No associations were noted for richness, evenness, or enterotypes. Conclusions: Based on our results, some type of distortion of the gut bacteriome appears to be a global feature of T1D, and our findings for four distant populations add new candidates to the existing list of bacteria. It remains to be established whether the observed associations are markers or causative factors. (C) 2018 Elsevier B.V. All rights reserved.

Název v anglickém jazyce

The bacteriome at the onset of type 1 diabetes: A study from four geographically distant African and Asian countries

Popis výsledku anglicky

Objectives: Gut bacteriome profiling studies in type 1 diabetes (T1D) to date are mostly limited to populations of Europe, with two studies from China and one study each from Mexico and the USA. We therefore sought to characterize the stool bacteriome in children after onset of T1D along with age-and place-matched control subjects from four geographically distant African and Asian countries. Methods: Samples were collected from 73 children and adolescents shortly after T1D onset (Azerbaijan 19, Jordan 20, Nigeria 14, Sudan 20) and 104 matched control subjects of similar age and locale. Genotyping of major T1D susceptibility genes was performed using saliva or blood samples. The bacteriome was profiled by next-generation sequencing of 16S rDNA. Negative binomial regression was used to model associations, with adjustment for the matched structure of the study. Results: A significant positive association with T1D was noted for the genus Escherichia (class Gammaproteobacteria, phylum Proteobacteria), whereas Eubacterium and Roseburia, two genera of class Clostridia, phylum Firmicutes, were inversely associated with T1D. We also confirmed a previously observed inverse association with Clostridium clusters IV or XIVa. No associations were noted for richness, evenness, or enterotypes. Conclusions: Based on our results, some type of distortion of the gut bacteriome appears to be a global feature of T1D, and our findings for four distant populations add new candidates to the existing list of bacteria. It remains to be established whether the observed associations are markers or causative factors. (C) 2018 Elsevier B.V. All rights reserved.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

30209 - Paediatrics

Projekt

<a href="/cs/project/NV15-31426A" target="_blank" >NV15-31426A: Dětský diabetes v subsaharské Africe: genetické a virologické nálezy a jejich srovnání s evropskými populacemi</a><br>

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2018

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Diabetes Research and Clinical Practice

ISSN

0168-8227

e-ISSN

—

Svazek periodika

144

Číslo periodika v rámci svazku

October

Stát vydavatele periodika

IE - Irsko

Počet stran výsledku

12

Strana od-do

51-62

Kód UT WoS článku

000447746400007

EID výsledku v databázi Scopus

2-s2.0-85052001205