Skin wipe test: A simple, inexpensive, and fast approach in the diagnosis of cystic fibrosis

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11130%2F20%3A10411271" target="_blank" >RIV/00216208:11130/20:10411271 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00216224:14740/20:00116334 RIV/65269705:_____/20:00072820 RIV/00064203:_____/20:10411271

Výsledek na webu

<a href="https://verso.is.cuni.cz/pub/verso.fpl?fname=obd_publikace_handle&handle=cEkRQ5Ct3~" target="_blank" >https://verso.is.cuni.cz/pub/verso.fpl?fname=obd_publikace_handle&handle=cEkRQ5Ct3~</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1002/ppul.24758" target="_blank" >10.1002/ppul.24758</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Skin wipe test: A simple, inexpensive, and fast approach in the diagnosis of cystic fibrosis

Popis výsledku v původním jazyce

Objective: To assess the performance of a newly developed skin wipe test (SWT) for the diagnosis of cystic fibrosis (CF). Study Design: Spontaneously formed sweat from the forearm was wiped by a cotton swab moistened with 100 µL of deionized (DI) water and extracted into 400 µL of DI water (SWT). The conventional Macroduct sweat test (ST) was performed simultaneously. SWT samples of 114 CF patients, 76 healthy carriers, and 58 controls were analyzed by capillary electrophoresis with contactless conductivity detection and Cl-/K+ and (Cl- + Na+)/K+ ion ratios were evaluated. Chloride concentrations from Macroduct ST were analyzed coulometrically. Results: Analysis of 248 SWT samples and simultaneous Macroduct ST samples showed comparable method performance. Two ion ratios, Cl-/K+ and (Cl- + Na+)/K+, from the SWT samples and Cl- values from the ST samples were evaluated to diagnose CF. Sensitivity of the SWT method using the Cl-/K+ ratio (cutoff value 3.9) was 93.9%, compared to 99.1% when using the (Cl- + Na+)/K+ ratio (cutoff value 5.0) and 98.3% in using Macroduct Cl- (cutoff value higher or equal to 60 mmol/L). The methods&apos; specificities were 97.8%, 94.0%, and 100.0%, respectively. Conclusions: The developed SWT method with capillary electrophoretic analysis for CF diagnosis performs comparably to the conventional Macroduct ST. The SWT method is simple, fast, inexpensive, and completely noninvasive. Use of an ion ratio in obtained SWT samples is proposed as a new diagnostic parameter that shows significant promise in CF diagnostics.

Název v anglickém jazyce

Skin wipe test: A simple, inexpensive, and fast approach in the diagnosis of cystic fibrosis

Popis výsledku anglicky

Objective: To assess the performance of a newly developed skin wipe test (SWT) for the diagnosis of cystic fibrosis (CF). Study Design: Spontaneously formed sweat from the forearm was wiped by a cotton swab moistened with 100 µL of deionized (DI) water and extracted into 400 µL of DI water (SWT). The conventional Macroduct sweat test (ST) was performed simultaneously. SWT samples of 114 CF patients, 76 healthy carriers, and 58 controls were analyzed by capillary electrophoresis with contactless conductivity detection and Cl-/K+ and (Cl- + Na+)/K+ ion ratios were evaluated. Chloride concentrations from Macroduct ST were analyzed coulometrically. Results: Analysis of 248 SWT samples and simultaneous Macroduct ST samples showed comparable method performance. Two ion ratios, Cl-/K+ and (Cl- + Na+)/K+, from the SWT samples and Cl- values from the ST samples were evaluated to diagnose CF. Sensitivity of the SWT method using the Cl-/K+ ratio (cutoff value 3.9) was 93.9%, compared to 99.1% when using the (Cl- + Na+)/K+ ratio (cutoff value 5.0) and 98.3% in using Macroduct Cl- (cutoff value higher or equal to 60 mmol/L). The methods&apos; specificities were 97.8%, 94.0%, and 100.0%, respectively. Conclusions: The developed SWT method with capillary electrophoretic analysis for CF diagnosis performs comparably to the conventional Macroduct ST. The SWT method is simple, fast, inexpensive, and completely noninvasive. Use of an ion ratio in obtained SWT samples is proposed as a new diagnostic parameter that shows significant promise in CF diagnostics.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

30209 - Paediatrics

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2020

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Pediatric Pulmonology

ISSN

8755-6863

e-ISSN

—

Svazek periodika

55

Číslo periodika v rámci svazku

7

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

8

Strana od-do

1653-1660

Kód UT WoS článku

000559584700001

EID výsledku v databázi Scopus

2-s2.0-85085076847