The effect of alternative solvents on the biocompatibility of centrifugally spun poly-ε-caprolactone

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11130%2F20%3A10412734" target="_blank" >RIV/00216208:11130/20:10412734 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00216208:11310/20:10412734 RIV/68378041:_____/20:00577513

Výsledek na webu

<a href="https://doi.org/10.4028/www.scientific.net/KEM.834.155" target="_blank" >https://doi.org/10.4028/www.scientific.net/KEM.834.155</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.4028/www.scientific.net/KEM.834.155" target="_blank" >10.4028/www.scientific.net/KEM.834.155</a>

Jazyk výsledku

angličtina

Název v původním jazyce

The effect of alternative solvents on the biocompatibility of centrifugally spun poly-ε-caprolactone

Popis výsledku v původním jazyce

Suitable scaffolds for tissue engineering should promote several features that enable regeneration of the damaged tissue in vivo. In general, nano- to microfibrous meshes resemble extracellular matrix and support cell adhesion; three dimensional scaffolds, together with interconnected pores, promote cell migration into the volume of the scaffolds. Furthermore, the scaffold should be biodegradable with no harmful byproducts and easy to produce. Centrifugal spinning is an alternative method, to widely used electrospinning method, to produce 3D scaffolds suitable for use in tissue engineering. In this study, we tested different molecular weights and solvent systems of poly-ε-caprolactone (PCL) that were produced by the centrifugal spinning method. The produced scaffolds were characterized and seeded with Saos2 osteosarcoma cell line to verify their biocompatibility. We concluded from the results that group 4 scaffold, produced from a mixture of two molecular weights of PCL dissolved in acetic acid/formic acid, supported cell adhesion, proliferation and metabolic activity the most out of all the tested scaffolds. The other PCL scaffolds were prepared either from one type of molecular weight of PCL or chloroform was solely used to produce the scaffolds.

Název v anglickém jazyce

The effect of alternative solvents on the biocompatibility of centrifugally spun poly-ε-caprolactone

Popis výsledku anglicky

Suitable scaffolds for tissue engineering should promote several features that enable regeneration of the damaged tissue in vivo. In general, nano- to microfibrous meshes resemble extracellular matrix and support cell adhesion; three dimensional scaffolds, together with interconnected pores, promote cell migration into the volume of the scaffolds. Furthermore, the scaffold should be biodegradable with no harmful byproducts and easy to produce. Centrifugal spinning is an alternative method, to widely used electrospinning method, to produce 3D scaffolds suitable for use in tissue engineering. In this study, we tested different molecular weights and solvent systems of poly-ε-caprolactone (PCL) that were produced by the centrifugal spinning method. The produced scaffolds were characterized and seeded with Saos2 osteosarcoma cell line to verify their biocompatibility. We concluded from the results that group 4 scaffold, produced from a mixture of two molecular weights of PCL dissolved in acetic acid/formic acid, supported cell adhesion, proliferation and metabolic activity the most out of all the tested scaffolds. The other PCL scaffolds were prepared either from one type of molecular weight of PCL or chloroform was solely used to produce the scaffolds.

Druh

C - Kapitola v odborné knize

CEP obor

—

OECD FORD obor

10610 - Biophysics

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2020

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název knihy nebo sborníku

Key Engineering Materials

ISBN

978-3-0357-1519-4

Počet stran výsledku

7

Strana od-do

155-161

Počet stran knihy

182

Název nakladatele

Trans Tech Publications Ltd

Místo vydání

Švýcarsko

Kód UT WoS kapitoly

—