The Effect of Alternative Solvents on the Biocompatibility of Centrifugally Spun Poly-ε-Caprolactone

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68407700%3A21720%2F20%3A00346721" target="_blank" >RIV/68407700:21720/20:00346721 - isvavai.cz</a>

Výsledek na webu

<a href="https://doi.org/10.4028/www.scientific.net/KEM.834.155" target="_blank" >https://doi.org/10.4028/www.scientific.net/KEM.834.155</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.4028/www.scientific.net/KEM.834.155" target="_blank" >10.4028/www.scientific.net/KEM.834.155</a>

Jazyk výsledku

angličtina

Název v původním jazyce

The Effect of Alternative Solvents on the Biocompatibility of Centrifugally Spun Poly-ε-Caprolactone

Popis výsledku v původním jazyce

Suitable scaffolds for tissue engineering should promote several features that enable regeneration of the damaged tissue in vivo. In general, nano- to microfibrous meshes resemble extracellular matrix and support cell adhesion; three dimensional scaffolds, together with interconnected pores, promote cell migration into the volume of the scaffolds. Furthermore, the scaffold should be biodegradable with no harmful byproducts and easy to produce. Centrifugal spinning is an alternative method, to widely used electrospinning method, to produce 3D scaffolds suitable for use in tissue engineering. In this study, we tested different molecular weights and solvent systems of poly-ε-caprolactone (PCL) that were produced by the centrifugal spinning method. The produced scaffolds were characterized and seeded with Saos2 osteosarcoma cell line to verify their biocompatibility. We concluded from the results that group 4 scaffold, produced from a mixture of two molecular weights of PCL dissolved in acetic acid/formic acid, supported cell adhesion, proliferation and metabolic activity the most out of all the tested scaffolds. The other PCL scaffolds were prepared either from one type of molecular weight of PCL or chloroform was solely used to produce the scaffolds.

Název v anglickém jazyce

The Effect of Alternative Solvents on the Biocompatibility of Centrifugally Spun Poly-ε-Caprolactone

Popis výsledku anglicky

Suitable scaffolds for tissue engineering should promote several features that enable regeneration of the damaged tissue in vivo. In general, nano- to microfibrous meshes resemble extracellular matrix and support cell adhesion; three dimensional scaffolds, together with interconnected pores, promote cell migration into the volume of the scaffolds. Furthermore, the scaffold should be biodegradable with no harmful byproducts and easy to produce. Centrifugal spinning is an alternative method, to widely used electrospinning method, to produce 3D scaffolds suitable for use in tissue engineering. In this study, we tested different molecular weights and solvent systems of poly-ε-caprolactone (PCL) that were produced by the centrifugal spinning method. The produced scaffolds were characterized and seeded with Saos2 osteosarcoma cell line to verify their biocompatibility. We concluded from the results that group 4 scaffold, produced from a mixture of two molecular weights of PCL dissolved in acetic acid/formic acid, supported cell adhesion, proliferation and metabolic activity the most out of all the tested scaffolds. The other PCL scaffolds were prepared either from one type of molecular weight of PCL or chloroform was solely used to produce the scaffolds.

Druh

D - Stať ve sborníku

CEP obor

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OECD FORD obor

30213 - Transplantation

Projekt

<a href="/cs/project/NV17-32285A" target="_blank" >NV17-32285A: Funkcionalizovaná nanovlákna v prevenci vzniku kýly v jizvě</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2020

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název statě ve sborníku

Key Engineering Materials Vol. 834

ISBN

9783035715194

ISSN

1013-9826

e-ISSN

—

Počet stran výsledku

7

Strana od-do

155-161

Název nakladatele

Trans Tech Publications Ltd.

Místo vydání

Pfaffikon

Místo konání akce

Salt lake city

Datum konání akce

2. 8. 2019

Typ akce podle státní příslušnosti

WRD - Celosvětová akce

Kód UT WoS článku

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