Surface Microdialysis for Detection of Colorectal Anastomosis Ischemia—An Experimental Study
Identifikátory výsledku
Kód výsledku v IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11130%2F21%3A10458381" target="_blank" >RIV/00216208:11130/21:10458381 - isvavai.cz</a>
Nalezeny alternativní kódy
RIV/67985904:_____/21:00541962 RIV/00023001:_____/21:00080673
Výsledek na webu
<a href="https://verso.is.cuni.cz/pub/verso.fpl?fname=obd_publikace_handle&handle=9xCcw-P5WX" target="_blank" >https://verso.is.cuni.cz/pub/verso.fpl?fname=obd_publikace_handle&handle=9xCcw-P5WX</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.jss.2020.12.021" target="_blank" >10.1016/j.jss.2020.12.021</a>
Alternativní jazyky
Jazyk výsledku
angličtina
Název v původním jazyce
Surface Microdialysis for Detection of Colorectal Anastomosis Ischemia—An Experimental Study
Popis výsledku v původním jazyce
Background: Inadequate blood supply is one of the major risk factors for anastomotic leak after low anterior rectal resection. Early detection of ischemia might predict complicated healing and enable anastomotic salvage, which is associated with better outcomes. A microdialysis offers a real-time evaluation of adequate bowel perfusion through moni-toring of tissue metabolism. In this experimental study, we assessed the role of micro-dialysis in detecting early ischemia after colorectal anastomosis. Materials and methods: Colorectal anastomosis was performed in six miniature pigs. A microdialysis catheter was placed on the bowel serosa 5 mm proximal to the anastomosis. Metabolic changes were monitored hourly before and after initiating ischemia, which was induced by ligation of the inferior mesenteric artery and skeletonization of the mesocolon. Results: Significant increase in tissue levels of lactate was detected after 60 min of ischemia (13.6 [10.4-16.1] versus 6.75 [1.8-9.6] mmol/L at baseline; P < 0.005). The lactate/pyruvate ratio increased accordingly. The concentration of glycerol increased significantly after 2 hoursdfrom a baseline value of 29.5 (3-84) to 125 (79-201) mmol/L (P < 0.005). The decrease in glucose levels was also significant after 60 minutesd0 (0-0.2) versus 4.7 (3.3-6.8) mmol/L at baseline. However, its values started to decline before ischemia. Conclusions: Surface microdialysis can detect ischemic changes early and may be a prom-ising method in postoperative monitoring of colorectal anastomosis.
Název v anglickém jazyce
Surface Microdialysis for Detection of Colorectal Anastomosis Ischemia—An Experimental Study
Popis výsledku anglicky
Background: Inadequate blood supply is one of the major risk factors for anastomotic leak after low anterior rectal resection. Early detection of ischemia might predict complicated healing and enable anastomotic salvage, which is associated with better outcomes. A microdialysis offers a real-time evaluation of adequate bowel perfusion through moni-toring of tissue metabolism. In this experimental study, we assessed the role of micro-dialysis in detecting early ischemia after colorectal anastomosis. Materials and methods: Colorectal anastomosis was performed in six miniature pigs. A microdialysis catheter was placed on the bowel serosa 5 mm proximal to the anastomosis. Metabolic changes were monitored hourly before and after initiating ischemia, which was induced by ligation of the inferior mesenteric artery and skeletonization of the mesocolon. Results: Significant increase in tissue levels of lactate was detected after 60 min of ischemia (13.6 [10.4-16.1] versus 6.75 [1.8-9.6] mmol/L at baseline; P < 0.005). The lactate/pyruvate ratio increased accordingly. The concentration of glycerol increased significantly after 2 hoursdfrom a baseline value of 29.5 (3-84) to 125 (79-201) mmol/L (P < 0.005). The decrease in glucose levels was also significant after 60 minutesd0 (0-0.2) versus 4.7 (3.3-6.8) mmol/L at baseline. However, its values started to decline before ischemia. Conclusions: Surface microdialysis can detect ischemic changes early and may be a prom-ising method in postoperative monitoring of colorectal anastomosis.
Klasifikace
Druh
J<sub>imp</sub> - Článek v periodiku v databázi Web of Science
CEP obor
—
OECD FORD obor
30212 - Surgery
Návaznosti výsledku
Projekt
—
Návaznosti
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Ostatní
Rok uplatnění
2021
Kód důvěrnosti údajů
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Údaje specifické pro druh výsledku
Název periodika
Journal of Surgical Research
ISSN
0022-4804
e-ISSN
1095-8673
Svazek periodika
261
Číslo periodika v rámci svazku
May
Stát vydavatele periodika
US - Spojené státy americké
Počet stran výsledku
8
Strana od-do
293-300
Kód UT WoS článku
000635161100037
EID výsledku v databázi Scopus
2-s2.0-85099367416