How to: screening for mcr-mediated resistance to colistin

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11130%2F22%3A10432138" target="_blank" >RIV/00216208:11130/22:10432138 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00064203:_____/22:10432138

Výsledek na webu

<a href="https://verso.is.cuni.cz/pub/verso.fpl?fname=obd_publikace_handle&handle=FXY.ozKI5f" target="_blank" >https://verso.is.cuni.cz/pub/verso.fpl?fname=obd_publikace_handle&handle=FXY.ozKI5f</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.cmi.2021.09.009" target="_blank" >10.1016/j.cmi.2021.09.009</a>

Jazyk výsledku

angličtina

Název v původním jazyce

How to: screening for mcr-mediated resistance to colistin

Popis výsledku v původním jazyce

BACKGROUND: Colistin belongs to the last-resort antibiotics. The discovery of a plasmid-bound colistin resistance mediated by the mcr-gene(s) is of great concern since, given its biological potential, there is a risk of its rapid spread. OBJECTIVES: To discuss the current literature on the methods for the screening of mcr-mediated resistance to colistin. SOURCES: Literature was drawn from a search of PubMed between 1 January 2016 to 26 April 2021. CONTENT: The selective culture-based or culture-independent approach can be used for the screening of mcr-mediated resistance to colistin in clinical samples. Rapid Polymyxin NP, Colistin Drop or Colistin Agar Spot tests are applicable for the selection of isolates with a suspected resistance to colistin that has to be confimed by broth microdilution. The mcr-mediated resistance to colistin can be confirmed by the detection of the causal gene(s) or by phenotype using the EDTA-colistin broth disk elution; the production of the MCR-1 enzyme can be confirmed with lateral flow immunoassay, using MALDI-TOF or Liquid chromatography-based mass spectrometry. Whole genome sequencing (WGS) is the ultimate typing method. When a WGS platform is not available at a healthcare facility, a WGS-outsourced service, in combination with freely available bioinformatics tools, allows for the characterisation of the mcr-gene(s) carrying isolates. IMPLICATIONS: Mcr-mediated colistin resistance should be monitored through active targeted screening.The broth microdilution method is required for colistin susceptibility testing but as only a selected number of clinical isolates are tested, colistin resistance, including mcr-mediated, may remain undetected. In mcr-1 positive E. coli isolates, the minimal inhibitory concentration (MIC) to colistin can range from 2-8 mg/L, thus it is proposed that Enterobacterales with a colistin MIC of 2 mg/L should be also included in the mcr-mediated colistin resistance screening and those with a confirmed mcr-genotype and/or MCR-phenotype should be considered to be colistin-resistant.

Název v anglickém jazyce

How to: screening for mcr-mediated resistance to colistin

Popis výsledku anglicky

BACKGROUND: Colistin belongs to the last-resort antibiotics. The discovery of a plasmid-bound colistin resistance mediated by the mcr-gene(s) is of great concern since, given its biological potential, there is a risk of its rapid spread. OBJECTIVES: To discuss the current literature on the methods for the screening of mcr-mediated resistance to colistin. SOURCES: Literature was drawn from a search of PubMed between 1 January 2016 to 26 April 2021. CONTENT: The selective culture-based or culture-independent approach can be used for the screening of mcr-mediated resistance to colistin in clinical samples. Rapid Polymyxin NP, Colistin Drop or Colistin Agar Spot tests are applicable for the selection of isolates with a suspected resistance to colistin that has to be confimed by broth microdilution. The mcr-mediated resistance to colistin can be confirmed by the detection of the causal gene(s) or by phenotype using the EDTA-colistin broth disk elution; the production of the MCR-1 enzyme can be confirmed with lateral flow immunoassay, using MALDI-TOF or Liquid chromatography-based mass spectrometry. Whole genome sequencing (WGS) is the ultimate typing method. When a WGS platform is not available at a healthcare facility, a WGS-outsourced service, in combination with freely available bioinformatics tools, allows for the characterisation of the mcr-gene(s) carrying isolates. IMPLICATIONS: Mcr-mediated colistin resistance should be monitored through active targeted screening.The broth microdilution method is required for colistin susceptibility testing but as only a selected number of clinical isolates are tested, colistin resistance, including mcr-mediated, may remain undetected. In mcr-1 positive E. coli isolates, the minimal inhibitory concentration (MIC) to colistin can range from 2-8 mg/L, thus it is proposed that Enterobacterales with a colistin MIC of 2 mg/L should be also included in the mcr-mediated colistin resistance screening and those with a confirmed mcr-genotype and/or MCR-phenotype should be considered to be colistin-resistant.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

30303 - Infectious Diseases

Projekt

<a href="/cs/project/NV18-09-00254" target="_blank" >NV18-09-00254: Sentinelová studie výskytu na plazmid vázané (přenositelné) rezistence ke kolistinu u Enterobacteriaceae v České republice</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2022

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Clinical Microbiology and Infection

ISSN

1198-743X

e-ISSN

1469-0691

Svazek periodika

28

Číslo periodika v rámci svazku

1

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

8

Strana od-do

43-50

Kód UT WoS článku

000735207100007

EID výsledku v databázi Scopus

2-s2.0-85121445328