Technical Note: Rapid Species Barcoding Using Bento Lab Mobile Laboratory

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11130%2F24%3A10489593" target="_blank" >RIV/00216208:11130/24:10489593 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00216208:11310/24:10489593

Výsledek na webu

<a href="https://verso.is.cuni.cz/pub/verso.fpl?fname=obd_publikace_handle&handle=4oqwi_1CK1" target="_blank" >https://verso.is.cuni.cz/pub/verso.fpl?fname=obd_publikace_handle&handle=4oqwi_1CK1</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.3390/forensicsci4040038" target="_blank" >10.3390/forensicsci4040038</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Technical Note: Rapid Species Barcoding Using Bento Lab Mobile Laboratory

Popis výsledku v původním jazyce

In scenarios when the morphological identification of samples is not feasible, the utilization of molecular analyses becomes an imperative. However, that can often be challenging in conditions where standard molecular laboratories cannot be established. In this study, we present a simplified and field-friendly protocol of the previously published Triplex PCR Assay for the identification of mammalian and plant sample origin using a fully portable molecular laboratory from Bento Lab (Bento Bioworks Ltd., London, UK), effectively eliminating the need for a traditional laboratory setup. The protocol in combination with correctly selected kits enables DNA extraction, result evaluation based on electrophoresis, and direct use of the PCR products for downstream analyses such as Sanger sequencing and third-generation sequencing; therefore, enabling the use of molecular analysis directly in situ or for educational purposes in a classroom.

Název v anglickém jazyce

Technical Note: Rapid Species Barcoding Using Bento Lab Mobile Laboratory

Popis výsledku anglicky

In scenarios when the morphological identification of samples is not feasible, the utilization of molecular analyses becomes an imperative. However, that can often be challenging in conditions where standard molecular laboratories cannot be established. In this study, we present a simplified and field-friendly protocol of the previously published Triplex PCR Assay for the identification of mammalian and plant sample origin using a fully portable molecular laboratory from Bento Lab (Bento Bioworks Ltd., London, UK), effectively eliminating the need for a traditional laboratory setup. The protocol in combination with correctly selected kits enables DNA extraction, result evaluation based on electrophoresis, and direct use of the PCR products for downstream analyses such as Sanger sequencing and third-generation sequencing; therefore, enabling the use of molecular analysis directly in situ or for educational purposes in a classroom.

Druh

J_SC - Článek v periodiku v databázi SCOPUS

CEP obor

—

OECD FORD obor

10511 - Environmental sciences (social aspects to be 5.7)

Projekt

<a href="/cs/project/VJ01010026" target="_blank" >VJ01010026: Efektivní využívání forenzních metod dokazování v oblasti boje proti wildlife crime</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2024

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Forensic Sciences (Basel)

ISSN

2673-6756

e-ISSN

2673-6756

Svazek periodika

4

Číslo periodika v rámci svazku

4

Stát vydavatele periodika

CH - Švýcarská konfederace

Počet stran výsledku

7

Strana od-do

566-572

Kód UT WoS článku

—

EID výsledku v databázi Scopus

2-s2.0-85213495933