Pixe maps of intracellular element distribution in cerebellar neurons

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11140%2F12%3A10125478" target="_blank" >RIV/00216208:11140/12:10125478 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/61389005:_____/12:00388232

Výsledek na webu

<a href="http://dx.doi.org/10.1142/S0129083512400190" target="_blank" >http://dx.doi.org/10.1142/S0129083512400190</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1142/S0129083512400190" target="_blank" >10.1142/S0129083512400190</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Pixe maps of intracellular element distribution in cerebellar neurons

Popis výsledku v původním jazyce

Neurodegenerative disorders are accompanied by altered compositions of metals that may convey some information about the health and possibly metabolic state of neurons. Here we opted for PIXE technique to perform a spatially-resolved elemental analysis of intracellular space of individual neurons. Primarily, we measured element distribution within Purkinje cells (PC) of normal and Lurcher (Lc/+) mutant mice to detect any anomalies in concentrations of individual elements attributable to cell death. Lc/+mutant mice provide an excellent animal model for time-course of excitotoxic cell death. PCs, some with autofluorescence, were scanned with a proton beam to record their X-ray emission and RBS spectra and to construct 2D-concentration maps of individualelements. PCs with auto-fluorescence, presumed to be either already dead or moribund, showed elevated concentrations of Cu, Fe, and Zn, when compared to healthy PCs. In conclusion, excitotoxic mode of death, as observed in the moribund P

Název v anglickém jazyce

Pixe maps of intracellular element distribution in cerebellar neurons

Popis výsledku anglicky

Neurodegenerative disorders are accompanied by altered compositions of metals that may convey some information about the health and possibly metabolic state of neurons. Here we opted for PIXE technique to perform a spatially-resolved elemental analysis of intracellular space of individual neurons. Primarily, we measured element distribution within Purkinje cells (PC) of normal and Lurcher (Lc/+) mutant mice to detect any anomalies in concentrations of individual elements attributable to cell death. Lc/+mutant mice provide an excellent animal model for time-course of excitotoxic cell death. PCs, some with autofluorescence, were scanned with a proton beam to record their X-ray emission and RBS spectra and to construct 2D-concentration maps of individualelements. PCs with auto-fluorescence, presumed to be either already dead or moribund, showed elevated concentrations of Cu, Fe, and Zn, when compared to healthy PCs. In conclusion, excitotoxic mode of death, as observed in the moribund P

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

FP - Ostatní lékařské obory

OECD FORD obor

—

Projekt

<a href="/cs/project/GA309%2F09%2F1189" target="_blank" >GA309/09/1189: Prvková analýza vnitrobuněčných struktur neuronů.</a><br>

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2012

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

International Journal of PIXE

ISSN

0129-0835

e-ISSN

—

Svazek periodika

22

Číslo periodika v rámci svazku

1a2

Stát vydavatele periodika

JP - Japonsko

Počet stran výsledku

8

Strana od-do

65-72

Kód UT WoS článku

—

EID výsledku v databázi Scopus

—