Cerebellar degeneration in lurcher mice under confocal laser scanning microscope

Kód výsledku v IS VaVaI

RIV/00216208:11140/13:10134171 - isvavai.cz

Výsledek na webu

http://onlinelibrary.wiley.com/doi/10.1002/jemt.22198/pdf

DOI - Digital Object Identifier

10.1002/jemt.22198

Jazyk výsledku

angličtina

Název v původním jazyce

Cerebellar degeneration in lurcher mice under confocal laser scanning microscope

Popis výsledku v původním jazyce

Lurcher mutant mice represent a natural model of genetically-determined olivocerebellar degeneration caused by a mutation in the ?2 glutamate receptor gene. They suffer from progressive postnatal loss of cerebellar Purkinje cells and a decrease of granule cells and inferior olive neurons. Their wild type littermates serve as healthy controls. A confocal laser scanning microscope was used aiming investigation the dynamics of changes in the cerebellar cortex of Lurcher and wild type mice derived from twostrains during the period of 8-21 postnatal days. Fluorescent double-staining was used to visualize mainly the Purkinje cells in cerebellar slices. In wild types, only normal Purkinje cells of round or regular drop-shaped were present, when staining intensity of other individual cell structures differed in dependence on the age of the animal. In Lurcher mutants, there were still some normal-shaped cells. Nevertheless, depending on the animal's age, a wide variety of stages of the cell de

Název v anglickém jazyce

Cerebellar degeneration in lurcher mice under confocal laser scanning microscope

Popis výsledku anglicky

Lurcher mutant mice represent a natural model of genetically-determined olivocerebellar degeneration caused by a mutation in the ?2 glutamate receptor gene. They suffer from progressive postnatal loss of cerebellar Purkinje cells and a decrease of granule cells and inferior olive neurons. Their wild type littermates serve as healthy controls. A confocal laser scanning microscope was used aiming investigation the dynamics of changes in the cerebellar cortex of Lurcher and wild type mice derived from twostrains during the period of 8-21 postnatal days. Fluorescent double-staining was used to visualize mainly the Purkinje cells in cerebellar slices. In wild types, only normal Purkinje cells of round or regular drop-shaped were present, when staining intensity of other individual cell structures differed in dependence on the age of the animal. In Lurcher mutants, there were still some normal-shaped cells. Nevertheless, depending on the animal's age, a wide variety of stages of the cell de

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

EA - Morfologické obory a cytologie

OECD FORD obor

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Projekt

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Návaznosti

S - Specificky vyzkum na vysokych skolach
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2013

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Microscopy Research and Technique

ISSN

1059-910X

e-ISSN

—

Svazek periodika

76

Číslo periodika v rámci svazku

5

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

7

Strana od-do

545-551

Kód UT WoS článku

000317299500014

EID výsledku v databázi Scopus

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