In vitro testing of immunosupressive effects of mesenchymal stromal cells on lymphocytes stimulated with alloantigens
Identifikátory výsledku
Kód výsledku v IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11140%2F15%3A10139963" target="_blank" >RIV/00216208:11140/15:10139963 - isvavai.cz</a>
Nalezeny alternativní kódy
RIV/00669806:_____/15:10139963
Výsledek na webu
<a href="http://dx.doi.org/10.5507/bp.2013.072" target="_blank" >http://dx.doi.org/10.5507/bp.2013.072</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.5507/bp.2013.072" target="_blank" >10.5507/bp.2013.072</a>
Alternativní jazyky
Jazyk výsledku
angličtina
Název v původním jazyce
In vitro testing of immunosupressive effects of mesenchymal stromal cells on lymphocytes stimulated with alloantigens
Popis výsledku v původním jazyce
Aims. Mesenchymal stromal cells (MSC) derived from adult bone marrow or adipose tissue offer the potential to open a new frontier in medicine. MSC are involved in modulating immune response and tissue repair in vitro and in vivo. Experimental evidence and preliminary clinical studies have demonstrated that MSC exhibit an important immunomodulatory function in patients with graft versus host disease (GVHD) following allogeneic hematopoietic stem cell transplantation. The immunosuppressive properties of MSC have already been exploited in the clinical setting. However the precise mechanisms are being still investigated. Methods. We examined the immunosuppressive function of MSC by coculturing them with stimulated HLA incompatible allogeneic lymphocytes ina mixed lymphocyte culture test. The metabolic and proliferative activity of lymphocytes was determined by MTT test. Results. After stimulation with alloantigens the presence of MSC caused significant decrease of absorbance levels by 62%
Název v anglickém jazyce
In vitro testing of immunosupressive effects of mesenchymal stromal cells on lymphocytes stimulated with alloantigens
Popis výsledku anglicky
Aims. Mesenchymal stromal cells (MSC) derived from adult bone marrow or adipose tissue offer the potential to open a new frontier in medicine. MSC are involved in modulating immune response and tissue repair in vitro and in vivo. Experimental evidence and preliminary clinical studies have demonstrated that MSC exhibit an important immunomodulatory function in patients with graft versus host disease (GVHD) following allogeneic hematopoietic stem cell transplantation. The immunosuppressive properties of MSC have already been exploited in the clinical setting. However the precise mechanisms are being still investigated. Methods. We examined the immunosuppressive function of MSC by coculturing them with stimulated HLA incompatible allogeneic lymphocytes ina mixed lymphocyte culture test. The metabolic and proliferative activity of lymphocytes was determined by MTT test. Results. After stimulation with alloantigens the presence of MSC caused significant decrease of absorbance levels by 62%
Klasifikace
Druh
J<sub>x</sub> - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)
CEP obor
FD - Onkologie a hematologie
OECD FORD obor
—
Návaznosti výsledku
Projekt
<a href="/cs/project/ED2.1.00%2F03.0076" target="_blank" >ED2.1.00/03.0076: Biomedicínské centrum Lékařské fakulty v Plzni</a><br>
Návaznosti
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Ostatní
Rok uplatnění
2015
Kód důvěrnosti údajů
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Údaje specifické pro druh výsledku
Název periodika
Biomedical papers of the Medical Faculty of the University Palacky, Olomouc, CzechoSK
ISSN
1213-8118
e-ISSN
—
Svazek periodika
159
Číslo periodika v rámci svazku
2
Stát vydavatele periodika
CZ - Česká republika
Počet stran výsledku
5
Strana od-do
215-219
Kód UT WoS článku
000359128900008
EID výsledku v databázi Scopus
2-s2.0-84934290503