Smaller Absolute Quantities but Greater Relative Densities of Microvessels Are Associated with Cerebellar Degeneration in Lurcher Mice

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11140%2F16%3A10323497" target="_blank" >RIV/00216208:11140/16:10323497 - isvavai.cz</a>

Výsledek na webu

<a href="http://dx.doi.org/10.3389/fnana.2016.00035" target="_blank" >http://dx.doi.org/10.3389/fnana.2016.00035</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.3389/fnana.2016.00035" target="_blank" >10.3389/fnana.2016.00035</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Smaller Absolute Quantities but Greater Relative Densities of Microvessels Are Associated with Cerebellar Degeneration in Lurcher Mice

Popis výsledku v původním jazyce

Degenerative affections of nerve tissues are often accompanied by changes of vascularization. In this regard, not much is known about hereditary cerebellar degeneration. In this study, we compared the vascularity of the individual cerebellar components and the mesencephalon of three-month-old wild type mice (n=5) and Lurcher mutant mice, which represent a model of hereditary olivocerebellar degeneration (n=5). Paraformaldehyde-fixed brains were processed into 18-µm thick serial sections with random orientation. Microvessels were visualised using polyclonal rabbit anti-laminin antibodies. Then, the stacks comprised of three 5-µm thick optical sections were recorded using systematic uniform random sampling. Stereological assessment was conducted based on photodocumentation. We found that each of the cerebellar components has its own features of vascularity. The greatest number and length of vessels were found in the granular layer; the number of vessels was lower in the molecular layer, and the lowest number of vessels was observed in the cerebellar nuclei corresponding with their low volume. Nevertheless, the nuclei had the greatest density of blood vessels. The reduction of cerebellum volume in the Lurcher mice was accompanied by a reduction in vascularization in the individual cerebellar components, mainly in the cortex. Moreover, despite the lower density of microvessels in the Lurcher mice compared with the wild type mice, the relative density of microvessels in the cerebellar cortex and nuclei was greater in Lurcher mice. The complete primary morphometric data, in the form of continuous variables, is included as a supplement. Mapping of the cereballar and midbrain microvessels has explanatory potential for studies using mouse models of neurodegeneration.

Název v anglickém jazyce

Smaller Absolute Quantities but Greater Relative Densities of Microvessels Are Associated with Cerebellar Degeneration in Lurcher Mice

Popis výsledku anglicky

Degenerative affections of nerve tissues are often accompanied by changes of vascularization. In this regard, not much is known about hereditary cerebellar degeneration. In this study, we compared the vascularity of the individual cerebellar components and the mesencephalon of three-month-old wild type mice (n=5) and Lurcher mutant mice, which represent a model of hereditary olivocerebellar degeneration (n=5). Paraformaldehyde-fixed brains were processed into 18-µm thick serial sections with random orientation. Microvessels were visualised using polyclonal rabbit anti-laminin antibodies. Then, the stacks comprised of three 5-µm thick optical sections were recorded using systematic uniform random sampling. Stereological assessment was conducted based on photodocumentation. We found that each of the cerebellar components has its own features of vascularity. The greatest number and length of vessels were found in the granular layer; the number of vessels was lower in the molecular layer, and the lowest number of vessels was observed in the cerebellar nuclei corresponding with their low volume. Nevertheless, the nuclei had the greatest density of blood vessels. The reduction of cerebellum volume in the Lurcher mice was accompanied by a reduction in vascularization in the individual cerebellar components, mainly in the cortex. Moreover, despite the lower density of microvessels in the Lurcher mice compared with the wild type mice, the relative density of microvessels in the cerebellar cortex and nuclei was greater in Lurcher mice. The complete primary morphometric data, in the form of continuous variables, is included as a supplement. Mapping of the cereballar and midbrain microvessels has explanatory potential for studies using mouse models of neurodegeneration.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

FH - Neurologie, neurochirurgie, neurovědy

OECD FORD obor

—

Projekt

<a href="/cs/project/LO1503" target="_blank" >LO1503: BIOMEDIC</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2016

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Frontiers in Neuroanatomy

ISSN

1662-5129

e-ISSN

—

Svazek periodika

neuveden

Číslo periodika v rámci svazku

April

Stát vydavatele periodika

CH - Švýcarská konfederace

Počet stran výsledku

14

Strana od-do

—

Kód UT WoS článku

000374307000001

EID výsledku v databázi Scopus

2-s2.0-84966277483