Reduction of Microvessel Number and Length in the Cerebellum of Purkinje Cell Degeneration Mice

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11140%2F24%3A10459066" target="_blank" >RIV/00216208:11140/24:10459066 - isvavai.cz</a>

Výsledek na webu

<a href="https://verso.is.cuni.cz/pub/verso.fpl?fname=obd_publikace_handle&handle=VsR1t2mqMK" target="_blank" >https://verso.is.cuni.cz/pub/verso.fpl?fname=obd_publikace_handle&handle=VsR1t2mqMK</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1007/s12311-023-01556-y" target="_blank" >10.1007/s12311-023-01556-y</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Reduction of Microvessel Number and Length in the Cerebellum of Purkinje Cell Degeneration Mice

Popis výsledku v původním jazyce

Degenerative effects of nerve tissues are often accompanied by changes in vascularization. In this regard, knowledge about hereditary cerebellar degeneration is limited. In this study, we compared the vascularity of the individual cerebellar components of 3-month-old wild-type mice (n = 8) and Purkinje cell degeneration (pcd) mutant mice, which represent a model of hereditary cerebellar degeneration (n = 8). Systematic random samples of tissue sections were processed, and laminin was immunostained to visualize microvessels. A computer-assisted stereology system was used to quantify microvessel parameters including total number, total length, and associated densities in cerebellar layers. Our results in pcd mice revealed a 45% (p &lt; 0.01) reduction in the total volume of the cerebellum, a 28% (p &lt; 0.05) reduction in the total number of vessels and a lower total length, approaching 50% (p &lt; 0.001), compared to the control mice. In pcd mutants, cerebellar degeneration is accompanied by significant reduction in the microvascular network that is proportional to the cerebellar volume reduction therefore does not change density of in the cerebellar gray matter of pcd mice.

Název v anglickém jazyce

Reduction of Microvessel Number and Length in the Cerebellum of Purkinje Cell Degeneration Mice

Popis výsledku anglicky

Degenerative effects of nerve tissues are often accompanied by changes in vascularization. In this regard, knowledge about hereditary cerebellar degeneration is limited. In this study, we compared the vascularity of the individual cerebellar components of 3-month-old wild-type mice (n = 8) and Purkinje cell degeneration (pcd) mutant mice, which represent a model of hereditary cerebellar degeneration (n = 8). Systematic random samples of tissue sections were processed, and laminin was immunostained to visualize microvessels. A computer-assisted stereology system was used to quantify microvessel parameters including total number, total length, and associated densities in cerebellar layers. Our results in pcd mice revealed a 45% (p &lt; 0.01) reduction in the total volume of the cerebellum, a 28% (p &lt; 0.05) reduction in the total number of vessels and a lower total length, approaching 50% (p &lt; 0.001), compared to the control mice. In pcd mutants, cerebellar degeneration is accompanied by significant reduction in the microvascular network that is proportional to the cerebellar volume reduction therefore does not change density of in the cerebellar gray matter of pcd mice.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

30106 - Anatomy and morphology (plant science to be 1.6)

Projekt

—

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2024

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

The Cerebellum

ISSN

1473-4222

e-ISSN

1473-4230

Svazek periodika

23

Číslo periodika v rámci svazku

2

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

8

Strana od-do

471-478

Kód UT WoS článku

000971502100001

EID výsledku v databázi Scopus

2-s2.0-85152938567