Trends in gene expression changes during adipogenesis in human adipose derived mesenchymal stem cells under dichlorodiphenyldichloroethylene exposure

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11140%2F18%3A10385964" target="_blank" >RIV/00216208:11140/18:10385964 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/49777513:23520/18:43952786

Výsledek na webu

<a href="https://link.springer.com/content/pdf/10.1007%2Fs13273-018-0041-1.pdf" target="_blank" >https://link.springer.com/content/pdf/10.1007%2Fs13273-018-0041-1.pdf</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1007/s13273-018-0041-1" target="_blank" >10.1007/s13273-018-0041-1</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Trends in gene expression changes during adipogenesis in human adipose derived mesenchymal stem cells under dichlorodiphenyldichloroethylene exposure

Popis výsledku v původním jazyce

Exposure to lipophilic environmental pollutants has been explored as a risk factor of development of diabetes mellitus in obese. Adipose tissue is a reservoir of bioaccumulative lipophilic contaminants including p,pʹ-dichlorodiphenyldichloroethylene (DDE). Our aim was to analyze the effect of DDE (in concentrations 0.1 μM, 1 μM, and 10 μM) on adipocyte differentiation and insulin signalling pathway on in vitro adipogenic model of human adipose derived mesenchymal stem cells (hADMSC). The effect of DDE was monitored by analysis of expression (RT qPCR, Western blotting) of genes (INSR, LIPE, FASN, SREBP1, OCT4 and AKT2)involved in adipocyte differentiation and insulin signalling pathway including lipid metabolism on days 0, 4, 10, 21, 28 of differentiation.Our findings suggest that DDE exposure changes the differentiation of adipocytes, enhances the lipid metabolism and so may play a role in the development of obesity and metabolic diseases by affecting the insulin signalling pathway. The influence of DDE seems to be similar to the effect of insulin itself. However, further studies to elucidate the mechanism of action of DDE are necessary.

Název v anglickém jazyce

Trends in gene expression changes during adipogenesis in human adipose derived mesenchymal stem cells under dichlorodiphenyldichloroethylene exposure

Popis výsledku anglicky

Exposure to lipophilic environmental pollutants has been explored as a risk factor of development of diabetes mellitus in obese. Adipose tissue is a reservoir of bioaccumulative lipophilic contaminants including p,pʹ-dichlorodiphenyldichloroethylene (DDE). Our aim was to analyze the effect of DDE (in concentrations 0.1 μM, 1 μM, and 10 μM) on adipocyte differentiation and insulin signalling pathway on in vitro adipogenic model of human adipose derived mesenchymal stem cells (hADMSC). The effect of DDE was monitored by analysis of expression (RT qPCR, Western blotting) of genes (INSR, LIPE, FASN, SREBP1, OCT4 and AKT2)involved in adipocyte differentiation and insulin signalling pathway including lipid metabolism on days 0, 4, 10, 21, 28 of differentiation.Our findings suggest that DDE exposure changes the differentiation of adipocytes, enhances the lipid metabolism and so may play a role in the development of obesity and metabolic diseases by affecting the insulin signalling pathway. The influence of DDE seems to be similar to the effect of insulin itself. However, further studies to elucidate the mechanism of action of DDE are necessary.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

30304 - Public and environmental health

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2018

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Molecular and Cellular Toxicology

ISSN

1738-642X

e-ISSN

—

Svazek periodika

14

Číslo periodika v rámci svazku

4

Stát vydavatele periodika

KR - Korejská republika

Počet stran výsledku

11

Strana od-do

369-379

Kód UT WoS článku

000445653400003

EID výsledku v databázi Scopus

2-s2.0-85053836371