Genomic Insight of VIM-harboring IncA Plasmid from a Clinical ST69Escherichia coliStrain in Italy
Identifikátory výsledku
Kód výsledku v IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11140%2F20%3A10415780" target="_blank" >RIV/00216208:11140/20:10415780 - isvavai.cz</a>
Výsledek na webu
<a href="https://verso.is.cuni.cz/pub/verso.fpl?fname=obd_publikace_handle&handle=ItUXIqz8rf" target="_blank" >https://verso.is.cuni.cz/pub/verso.fpl?fname=obd_publikace_handle&handle=ItUXIqz8rf</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.3390/microorganisms8081232" target="_blank" >10.3390/microorganisms8081232</a>
Alternativní jazyky
Jazyk výsledku
angličtina
Název v původním jazyce
Genomic Insight of VIM-harboring IncA Plasmid from a Clinical ST69Escherichia coliStrain in Italy
Popis výsledku v původním jazyce
Background: VIM (Verona Integron-encoded Metallo-beta-lactamase) is a member of the Metallo-Beta-Lactamases (MBLs), and is able to hydrolyze all beta-lactams antibiotics, except for monobactams, and including carbapenems. Here we characterize a VIM-producing IncA plasmid isolated from a clinical ST69Escherichia colistrain from an Italian Long-Term Care Facility (LTCF) inpatient.Methods: An antimicrobial susceptibility test and conjugation assay were carried out, and the transferability of thebla(VIM-type)gene was confirmed in the transconjugant. Whole-genome sequencing (WGS) of the strain 550 was performed using the Sequel I platform. Genome assembly was performed using "Microbial Assembly". Genomic analysis was conducted by uploading the contigs to ResFinder and PlasmidFinder databases.Results:Assembly resulted in three complete circular contigs: the chromosome (4,962,700 bp), an IncA plasmid (p550_IncA_VIM_1; 162,608 bp), harboring genes coding for aminoglycoside resistance (aac(6 ')-Ib4,ant(3 '')-Ia,aph(3 '')-Ib,aph(3 ')-XV,aph(6)-Id), beta-lactam resistance (bla(SHV-12),bla(VIM-1)), macrolides resistance (mph(A)), phenicol resistance (catB2), quinolones resistance (qnrS1), sulphonamide resistance (sul1,sul2), and trimethoprim resistance (dfrA14), and an IncK/Z plasmid (p550_IncB_O_K_Z; 100,306 bp), free of antibiotic resistance genes.Conclusions:The increase in reports of IncA plasmids bearing different antimicrobial resistance genes highlights the overall important role of IncA plasmids in disseminating carbapenemase genes, with a preference for thebla(VIM-1)gene in Italy.
Název v anglickém jazyce
Genomic Insight of VIM-harboring IncA Plasmid from a Clinical ST69Escherichia coliStrain in Italy
Popis výsledku anglicky
Background: VIM (Verona Integron-encoded Metallo-beta-lactamase) is a member of the Metallo-Beta-Lactamases (MBLs), and is able to hydrolyze all beta-lactams antibiotics, except for monobactams, and including carbapenems. Here we characterize a VIM-producing IncA plasmid isolated from a clinical ST69Escherichia colistrain from an Italian Long-Term Care Facility (LTCF) inpatient.Methods: An antimicrobial susceptibility test and conjugation assay were carried out, and the transferability of thebla(VIM-type)gene was confirmed in the transconjugant. Whole-genome sequencing (WGS) of the strain 550 was performed using the Sequel I platform. Genome assembly was performed using "Microbial Assembly". Genomic analysis was conducted by uploading the contigs to ResFinder and PlasmidFinder databases.Results:Assembly resulted in three complete circular contigs: the chromosome (4,962,700 bp), an IncA plasmid (p550_IncA_VIM_1; 162,608 bp), harboring genes coding for aminoglycoside resistance (aac(6 ')-Ib4,ant(3 '')-Ia,aph(3 '')-Ib,aph(3 ')-XV,aph(6)-Id), beta-lactam resistance (bla(SHV-12),bla(VIM-1)), macrolides resistance (mph(A)), phenicol resistance (catB2), quinolones resistance (qnrS1), sulphonamide resistance (sul1,sul2), and trimethoprim resistance (dfrA14), and an IncK/Z plasmid (p550_IncB_O_K_Z; 100,306 bp), free of antibiotic resistance genes.Conclusions:The increase in reports of IncA plasmids bearing different antimicrobial resistance genes highlights the overall important role of IncA plasmids in disseminating carbapenemase genes, with a preference for thebla(VIM-1)gene in Italy.
Klasifikace
Druh
J<sub>imp</sub> - Článek v periodiku v databázi Web of Science
CEP obor
—
OECD FORD obor
10606 - Microbiology
Návaznosti výsledku
Projekt
Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.
Návaznosti
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Ostatní
Rok uplatnění
2020
Kód důvěrnosti údajů
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Údaje specifické pro druh výsledku
Název periodika
Microorganisms
ISSN
2076-2607
e-ISSN
—
Svazek periodika
8
Číslo periodika v rámci svazku
8
Stát vydavatele periodika
CH - Švýcarská konfederace
Počet stran výsledku
10
Strana od-do
1232
Kód UT WoS článku
000564701400001
EID výsledku v databázi Scopus
2-s2.0-85090368219