Insights into the Resistome and Phylogenomics of a ST195 Multidrug-Resistant Acinetobacter baumannii Clinical Isolate from the Czech Republic

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F65269705%3A_____%2F21%3A00075298" target="_blank" >RIV/65269705:_____/21:00075298 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00098892:_____/21:N0000014 RIV/62157124:16810/21:43879648 RIV/62157124:16270/21:43879648 RIV/61989592:15110/21:73612646

Výsledek na webu

<a href="https://www.mdpi.com/2075-1729/11/10/1079" target="_blank" >https://www.mdpi.com/2075-1729/11/10/1079</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.3390/life11101079" target="_blank" >10.3390/life11101079</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Insights into the Resistome and Phylogenomics of a ST195 Multidrug-Resistant Acinetobacter baumannii Clinical Isolate from the Czech Republic

Popis výsledku v původním jazyce

Increasing antimicrobial resistance in nosocomial pathogens, such as Acinetobacter baumannii, is becoming a serious threat to public health. It is necessary to detect beta-lactamase-producing microorganisms in clinical settings to be able to control the spread of carbapenem resistance. This study was conducted to evaluate the presence of beta-lactamases in a selected clinical isolate of A. baumannii of ST2(P)/ST195(Ox) and to characterize possible enzymes, as well as its beta-lactam resistome, using PCR and whole-genome sequencing analysis. PCR and sequencing confirmed that the isolate harbored five bla gene alleles, namely, bla(ADC-73), bla(TEM-1), bla(OXA-23), bla(OXA-58) and bla(OXA-66), as well as aminoglycosides, macrolides, sulfonamides and tetracyclines resistance determinants, which were either chromosomally and/or plasmid located. Furthermore, a gene order comparison using MAUVE alignment showed multiple changes compared with the clinical isolate of Malaysian A. baumannii AC30 genome and 76 regions with high homology. This study suggests that resistance to beta-lactams in this A. baumannii isolate is mainly due to an overproduction of beta-lactamases in combination with other resistance mechanism (efflux pump system).

Název v anglickém jazyce

Insights into the Resistome and Phylogenomics of a ST195 Multidrug-Resistant Acinetobacter baumannii Clinical Isolate from the Czech Republic

Popis výsledku anglicky

Increasing antimicrobial resistance in nosocomial pathogens, such as Acinetobacter baumannii, is becoming a serious threat to public health. It is necessary to detect beta-lactamase-producing microorganisms in clinical settings to be able to control the spread of carbapenem resistance. This study was conducted to evaluate the presence of beta-lactamases in a selected clinical isolate of A. baumannii of ST2(P)/ST195(Ox) and to characterize possible enzymes, as well as its beta-lactam resistome, using PCR and whole-genome sequencing analysis. PCR and sequencing confirmed that the isolate harbored five bla gene alleles, namely, bla(ADC-73), bla(TEM-1), bla(OXA-23), bla(OXA-58) and bla(OXA-66), as well as aminoglycosides, macrolides, sulfonamides and tetracyclines resistance determinants, which were either chromosomally and/or plasmid located. Furthermore, a gene order comparison using MAUVE alignment showed multiple changes compared with the clinical isolate of Malaysian A. baumannii AC30 genome and 76 regions with high homology. This study suggests that resistance to beta-lactams in this A. baumannii isolate is mainly due to an overproduction of beta-lactamases in combination with other resistance mechanism (efflux pump system).

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10606 - Microbiology

Projekt

<a href="/cs/project/NU20J-09-00040" target="_blank" >NU20J-09-00040: Celogenomové sekvenování a metagenomika jako nástroje k pochopení cest přenosu bakterií rezistentních k antibiotikům z nemocnic do prostředí</a><br>

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2021

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Life-Basel

ISSN

2075-1729

e-ISSN

—

Svazek periodika

11

Číslo periodika v rámci svazku

10

Stát vydavatele periodika

CH - Švýcarská konfederace

Počet stran výsledku

14

Strana od-do

1079

Kód UT WoS článku

000714615600001

EID výsledku v databázi Scopus

2-s2.0-85117712968