TEM-1 beta-lactamase as a source of resistance to sulbactam in clinical strains of Acinetobacter baumannii

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11310%2F13%3A10159322" target="_blank" >RIV/00216208:11310/13:10159322 - isvavai.cz</a>

Výsledek na webu

<a href="http://jac.oxfordjournals.org/content/68/12/2786" target="_blank" >http://jac.oxfordjournals.org/content/68/12/2786</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1093/jac/dkt275" target="_blank" >10.1093/jac/dkt275</a>

Jazyk výsledku

angličtina

Název v původním jazyce

TEM-1 beta-lactamase as a source of resistance to sulbactam in clinical strains of Acinetobacter baumannii

Popis výsledku v původním jazyce

Objectives: Sulbactam is well known to have clinically relevant intrinsic activity against Acinetobacter baumannii. Although secondary resistance to this drug has long been reported in acinetobacters, virtually nothing is known about its molecular basis.The aim of this study was to test the hypothesis that beta-lactamase TEM-1 is responsible for sulbactam resistance in A. baumannii. Methods: Seventeen clinical strains of A. baumannii were selected to represent different combinations of quantitative susceptibilities to sulbactam and molecular typing characteristics. The strains were screened by PCR for the presence of the bla(TEM-1) gene and its variants. Amplicons encompassing the bla(TEM) genes, including their promoters, were sequenced. The expression and copy number of the bla(TEM) genes were assessed using semi-quantitative real-time PCR. Transfer of the bla(TEM-1) gene into a susceptible A. baumannii strain was achieved by electroporation. Results: Six strains were negative for t

Název v anglickém jazyce

TEM-1 beta-lactamase as a source of resistance to sulbactam in clinical strains of Acinetobacter baumannii

Popis výsledku anglicky

Objectives: Sulbactam is well known to have clinically relevant intrinsic activity against Acinetobacter baumannii. Although secondary resistance to this drug has long been reported in acinetobacters, virtually nothing is known about its molecular basis.The aim of this study was to test the hypothesis that beta-lactamase TEM-1 is responsible for sulbactam resistance in A. baumannii. Methods: Seventeen clinical strains of A. baumannii were selected to represent different combinations of quantitative susceptibilities to sulbactam and molecular typing characteristics. The strains were screened by PCR for the presence of the bla(TEM-1) gene and its variants. Amplicons encompassing the bla(TEM) genes, including their promoters, were sequenced. The expression and copy number of the bla(TEM) genes were assessed using semi-quantitative real-time PCR. Transfer of the bla(TEM-1) gene into a susceptible A. baumannii strain was achieved by electroporation. Results: Six strains were negative for t

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

FN - Epidemiologie, infekční nemoci a klinická imunologie

OECD FORD obor

—

Projekt

—

Návaznosti

S - Specificky vyzkum na vysokych skolach

Rok uplatnění

2013

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Journal of Antimicrobial Chemotherapy

ISSN

0305-7453

e-ISSN

—

Svazek periodika

68

Číslo periodika v rámci svazku

12

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

6

Strana od-do

2786-2791

Kód UT WoS článku

000326980000011

EID výsledku v databázi Scopus

—