Identification of 50 class D beta-lactamases and 65 Acinetobacter-derived cephalosporinases in Acinetobacter spp.

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F75010330%3A_____%2F14%3A00010668" target="_blank" >RIV/75010330:_____/14:00010668 - isvavai.cz</a>

Výsledek na webu

<a href="http://aac.asm.org/content/58/2/936" target="_blank" >http://aac.asm.org/content/58/2/936</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1128/AAC.01261-13" target="_blank" >10.1128/AAC.01261-13</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Identification of 50 class D beta-lactamases and 65 Acinetobacter-derived cephalosporinases in Acinetobacter spp.

Popis výsledku v původním jazyce

Whole-genome sequencing of a collection of 103 Acinetobacter strains belonging to 22 validly named species and another 16 putative species allowed detection of genes for 50 new class D beta-lactamases and 65 new Acinetobacter-derived cephalosporinases (ADC). All oxacillinases (OXA) contained the three typical motifs of class D beta-lactamases, STFK, (F/Y) GN, and K(S/T) G. The phylogenetic tree drawn from the OXA sequences led to an increase in the number of OXA groups from 7 to 18. The topologies of the OXA and RpoB phylogenetic trees were similar, supporting the ancient acquisition of bla(OXA) genes by Acinetobacter species. The class D beta-lactamase genes appeared to be intrinsic to several species, such as Acinetobacter baumannii, Acinetobacter pittii, Acinetobacter calcoaceticus, and Acinetobacter lwoffii. Neither bla(OXA-40/143)-nor bla(OXA-58)-like genes were detected, and their origin remains therefore unknown. The phylogenetic tree analysis based on the alignment of the seque

Název v anglickém jazyce

Identification of 50 class D beta-lactamases and 65 Acinetobacter-derived cephalosporinases in Acinetobacter spp.

Popis výsledku anglicky

Whole-genome sequencing of a collection of 103 Acinetobacter strains belonging to 22 validly named species and another 16 putative species allowed detection of genes for 50 new class D beta-lactamases and 65 new Acinetobacter-derived cephalosporinases (ADC). All oxacillinases (OXA) contained the three typical motifs of class D beta-lactamases, STFK, (F/Y) GN, and K(S/T) G. The phylogenetic tree drawn from the OXA sequences led to an increase in the number of OXA groups from 7 to 18. The topologies of the OXA and RpoB phylogenetic trees were similar, supporting the ancient acquisition of bla(OXA) genes by Acinetobacter species. The class D beta-lactamase genes appeared to be intrinsic to several species, such as Acinetobacter baumannii, Acinetobacter pittii, Acinetobacter calcoaceticus, and Acinetobacter lwoffii. Neither bla(OXA-40/143)-nor bla(OXA-58)-like genes were detected, and their origin remains therefore unknown. The phylogenetic tree analysis based on the alignment of the seque

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

EE - Mikrobiologie, virologie

OECD FORD obor

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Projekt

<a href="/cs/project/GA13-26693S" target="_blank" >GA13-26693S: Genetická diverzita a fylogeneze rodu Acinetobacter v přírodních ekosystémech.</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2014

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Antimicrobial Agents and Chemotherapy

ISSN

0066-4804

e-ISSN

—

Svazek periodika

58

Číslo periodika v rámci svazku

2

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

14

Strana od-do

936-949

Kód UT WoS článku

000330637500039

EID výsledku v databázi Scopus

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