Hyperbaric Oxygen Enhances Collagen III Formation in Wound of ZDF Rat
Identifikátory výsledku
Kód výsledku v IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11140%2F21%3A10436262" target="_blank" >RIV/00216208:11140/21:10436262 - isvavai.cz</a>
Výsledek na webu
<a href="https://verso.is.cuni.cz/pub/verso.fpl?fname=obd_publikace_handle&handle=-FZjDXWjq3" target="_blank" >https://verso.is.cuni.cz/pub/verso.fpl?fname=obd_publikace_handle&handle=-FZjDXWjq3</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.33549/physiolres.934684" target="_blank" >10.33549/physiolres.934684</a>
Alternativní jazyky
Jazyk výsledku
angličtina
Název v původním jazyce
Hyperbaric Oxygen Enhances Collagen III Formation in Wound of ZDF Rat
Popis výsledku v původním jazyce
Diabetic foot ulcer (DFU) is a serious complication of diabetes and hyperbaric oxygen therapy (HBOT) is also considered in comprehensive treatment. The evidence supporting the use of HBOT in DFU treatment is controversial. The aim of this work was to introduce a DFU model in ZDF rat by creating a wound on the back of an animal and to investigate the effect of HBOT on the defect by macroscopic evaluation, quantitative histological evaluation of collagen (types I and III), evaluation of angiogenesis and determination of interleukin 6 (IL6) levels in the plasma. The study included 10 rats in the control group (CONT) and 10 in the HBOT group, who underwent HBOT in standard clinical regimen. Histological evaluation was performed on the 18th day after induction of defect. The results show that HBOT did not affect the macroscopic size of the defect nor IL6 plasma levels. A volume fraction of type I collagen was slightly increased by HBOT without reaching statistical significance (1.35 +/- 0.49 and 1.94 +/- 0.67 %, CONT and HBOT, respectively). In contrast, the collagen type III volume fraction was similar to 120 % higher in HBOT wounds (1.41 +/- 0.81 %) than in CONT ones (0.63 +/- 0.37 %; p=0.046). In addition, the ratio of the volume fraction of both collagens in the wound ((I+III)(w)) to the volume fraction of both collagens in the adjacent healthy skin ((I+III)(h)) was similar to 65 % higher in rats subjected to HBOT (8.9 +/- 3.07 vs. 5.38 +/- 1.86 %, HBOT and CONT, respectively; p=0.028). Vessels density (number per 1 mm(2)) was found to be higher in CONT vs. HBOT (206.5 +/- 41.8 and 124 +/- 28.2, respectively, p<0.001). Our study suggests that HBOT promotes collagen III formation and decreases the number of newly formed vessels at the early phases of healing.
Název v anglickém jazyce
Hyperbaric Oxygen Enhances Collagen III Formation in Wound of ZDF Rat
Popis výsledku anglicky
Diabetic foot ulcer (DFU) is a serious complication of diabetes and hyperbaric oxygen therapy (HBOT) is also considered in comprehensive treatment. The evidence supporting the use of HBOT in DFU treatment is controversial. The aim of this work was to introduce a DFU model in ZDF rat by creating a wound on the back of an animal and to investigate the effect of HBOT on the defect by macroscopic evaluation, quantitative histological evaluation of collagen (types I and III), evaluation of angiogenesis and determination of interleukin 6 (IL6) levels in the plasma. The study included 10 rats in the control group (CONT) and 10 in the HBOT group, who underwent HBOT in standard clinical regimen. Histological evaluation was performed on the 18th day after induction of defect. The results show that HBOT did not affect the macroscopic size of the defect nor IL6 plasma levels. A volume fraction of type I collagen was slightly increased by HBOT without reaching statistical significance (1.35 +/- 0.49 and 1.94 +/- 0.67 %, CONT and HBOT, respectively). In contrast, the collagen type III volume fraction was similar to 120 % higher in HBOT wounds (1.41 +/- 0.81 %) than in CONT ones (0.63 +/- 0.37 %; p=0.046). In addition, the ratio of the volume fraction of both collagens in the wound ((I+III)(w)) to the volume fraction of both collagens in the adjacent healthy skin ((I+III)(h)) was similar to 65 % higher in rats subjected to HBOT (8.9 +/- 3.07 vs. 5.38 +/- 1.86 %, HBOT and CONT, respectively; p=0.028). Vessels density (number per 1 mm(2)) was found to be higher in CONT vs. HBOT (206.5 +/- 41.8 and 124 +/- 28.2, respectively, p<0.001). Our study suggests that HBOT promotes collagen III formation and decreases the number of newly formed vessels at the early phases of healing.
Klasifikace
Druh
J<sub>imp</sub> - Článek v periodiku v databázi Web of Science
CEP obor
—
OECD FORD obor
10610 - Biophysics
Návaznosti výsledku
Projekt
Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.
Návaznosti
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Ostatní
Rok uplatnění
2021
Kód důvěrnosti údajů
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Údaje specifické pro druh výsledku
Název periodika
Physiological Research
ISSN
0862-8408
e-ISSN
—
Svazek periodika
70
Číslo periodika v rámci svazku
5
Stát vydavatele periodika
CZ - Česká republika
Počet stran výsledku
12
Strana od-do
787-798
Kód UT WoS článku
000729810300012
EID výsledku v databázi Scopus
2-s2.0-85122305226