Male SIRT1 insufficiency leads to sperm with decreased ability to hyperactivate and fertilize

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11140%2F22%3A10445459" target="_blank" >RIV/00216208:11140/22:10445459 - isvavai.cz</a>

Výsledek na webu

<a href="https://verso.is.cuni.cz/pub/verso.fpl?fname=obd_publikace_handle&handle=3fkbF-ocKU" target="_blank" >https://verso.is.cuni.cz/pub/verso.fpl?fname=obd_publikace_handle&handle=3fkbF-ocKU</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1111/rda.14172" target="_blank" >10.1111/rda.14172</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Male SIRT1 insufficiency leads to sperm with decreased ability to hyperactivate and fertilize

Popis výsledku v původním jazyce

Deficient sperm motility is a frequent cause of the age-related male sub-/ infertility. Since the protein sirtuin 1 (SIRT1) develops anti-aging action and participates in sperm motility and ATP synthesis in mitochondria, we investigated its role in the acquisition of hyperactivated motility during capacitation. For this, the dynamics of sperm subpopulations were studied, using males of Sirt1+/- heterozygous mutant mice. After 2 hr of capacitation, we observed reduced percentage of hyperactivated spermatozoa in Sirt1+/- males. Interestingly, prior to capacitation, Sirt1+/- spermatozoa showed higher mitochondrial superoxide levels, which could render mitochondrial injury and thereby motility defects. Accordingly, the fertilization rate of Sirt1+/- males after mating was decreased. We elucidated that SIRT1 male insufficiency underlies posterior sperm defects to hyperactivate during capacitation and propose Sirt1+/- males as a model for the study of the age-related infertility.

Název v anglickém jazyce

Male SIRT1 insufficiency leads to sperm with decreased ability to hyperactivate and fertilize

Popis výsledku anglicky

Deficient sperm motility is a frequent cause of the age-related male sub-/ infertility. Since the protein sirtuin 1 (SIRT1) develops anti-aging action and participates in sperm motility and ATP synthesis in mitochondria, we investigated its role in the acquisition of hyperactivated motility during capacitation. For this, the dynamics of sperm subpopulations were studied, using males of Sirt1+/- heterozygous mutant mice. After 2 hr of capacitation, we observed reduced percentage of hyperactivated spermatozoa in Sirt1+/- males. Interestingly, prior to capacitation, Sirt1+/- spermatozoa showed higher mitochondrial superoxide levels, which could render mitochondrial injury and thereby motility defects. Accordingly, the fertilization rate of Sirt1+/- males after mating was decreased. We elucidated that SIRT1 male insufficiency underlies posterior sperm defects to hyperactivate during capacitation and propose Sirt1+/- males as a model for the study of the age-related infertility.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

30105 - Physiology (including cytology)

Projekt

<a href="/cs/project/EF16_019%2F0000787" target="_blank" >EF16_019/0000787: Centrum výzkumu infekčních onemocnění</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>S - Specificky vyzkum na vysokych skolach<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2022

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Reproduction in Domestic Animals

ISSN

0936-6768

e-ISSN

1439-0531

Svazek periodika

57

Číslo periodika v rámci svazku

S5

Stát vydavatele periodika

DE - Spolková republika Německo

Počet stran výsledku

6

Strana od-do

72-77

Kód UT WoS článku

000810579100001

EID výsledku v databázi Scopus

2-s2.0-85131728778