Role of N-Cadherin in Epithelial-to-Mesenchymal Transition and Chemosensitivity of Colon Carcinoma Cells

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11140%2F22%3A10450913" target="_blank" >RIV/00216208:11140/22:10450913 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/62690094:18470/22:50019605 RIV/75010330:_____/22:00014083 RIV/00216208:11150/22:10450913 RIV/00216208:11160/22:10450913 RIV/00179906:_____/22:10450913

Výsledek na webu

<a href="https://verso.is.cuni.cz/pub/verso.fpl?fname=obd_publikace_handle&handle=FAwQG~LCUl" target="_blank" >https://verso.is.cuni.cz/pub/verso.fpl?fname=obd_publikace_handle&handle=FAwQG~LCUl</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.3390/cancers14205146" target="_blank" >10.3390/cancers14205146</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Role of N-Cadherin in Epithelial-to-Mesenchymal Transition and Chemosensitivity of Colon Carcinoma Cells

Popis výsledku v původním jazyce

Simple Summary The role of N-cadherin expression in epithelial-to-mesenchymal transition (EMT) and related aggressive tumor colon cancer cell phenotype was investigated using various in vitro and in vivo models. With the help of several standard laboratory techniques, it was verified that an artificially increased N-cadherin expression has only a limited reprogramming potential towards colon cancer cells unlike the case where colon cancer cells present with a naturally elevated presence of N-cadherin. (1) Background: N-cadherin expression, epithelial-to-mesenchymal transition (EMT) and aggressive biological phenotype of tumor cells are linked although the underlying mechanisms are not entirely clear. (2) Methods: In this study, we used two different in vitro cell models with varying N-cadherin expression (stabilized lines and primocultures) and investigated their select biological features including the degree of their chemoresistance both in vitro as well as in vivo. (3) Results: We report that although enforced N-cadherin expression changes select morphological and behavioral characteristics of exposed cells, it fails to successfully reprogram cells to the aggressive, chemoresistant phenotype both in vitro as well as in vivo as verified by implantation of those cells into athymic mice. Conversely, primocultures of patient-colonic cells with naturally high levels of N-cadherin expression show fully aggressive and chemoresistant phenotype pertinent to EMT (in vitro and in vivo), with a potential to develop new mutations and in the presence of dysregulated regulatory pathways as represented by investigated miRNA profiles. (4) Conclusions: The presented results bring new facts concerning the functional axis of N-cadherin expression and related biological features of colon cancer cells and highlight colon cancer primocultures as a useful model for such studies.

Název v anglickém jazyce

Role of N-Cadherin in Epithelial-to-Mesenchymal Transition and Chemosensitivity of Colon Carcinoma Cells

Popis výsledku anglicky

Simple Summary The role of N-cadherin expression in epithelial-to-mesenchymal transition (EMT) and related aggressive tumor colon cancer cell phenotype was investigated using various in vitro and in vivo models. With the help of several standard laboratory techniques, it was verified that an artificially increased N-cadherin expression has only a limited reprogramming potential towards colon cancer cells unlike the case where colon cancer cells present with a naturally elevated presence of N-cadherin. (1) Background: N-cadherin expression, epithelial-to-mesenchymal transition (EMT) and aggressive biological phenotype of tumor cells are linked although the underlying mechanisms are not entirely clear. (2) Methods: In this study, we used two different in vitro cell models with varying N-cadherin expression (stabilized lines and primocultures) and investigated their select biological features including the degree of their chemoresistance both in vitro as well as in vivo. (3) Results: We report that although enforced N-cadherin expression changes select morphological and behavioral characteristics of exposed cells, it fails to successfully reprogram cells to the aggressive, chemoresistant phenotype both in vitro as well as in vivo as verified by implantation of those cells into athymic mice. Conversely, primocultures of patient-colonic cells with naturally high levels of N-cadherin expression show fully aggressive and chemoresistant phenotype pertinent to EMT (in vitro and in vivo), with a potential to develop new mutations and in the presence of dysregulated regulatory pathways as represented by investigated miRNA profiles. (4) Conclusions: The presented results bring new facts concerning the functional axis of N-cadherin expression and related biological features of colon cancer cells and highlight colon cancer primocultures as a useful model for such studies.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

30204 - Oncology

Projekt

<a href="/cs/project/NV19-08-00113" target="_blank" >NV19-08-00113: Studie využitelnosti sekvenování nové generace pro individualizovanou léčbu pacientů se solidními nádory</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2022

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Cancers

ISSN

2072-6694

e-ISSN

2072-6694

Svazek periodika

14

Číslo periodika v rámci svazku

20

Stát vydavatele periodika

CH - Švýcarská konfederace

Počet stran výsledku

19

Strana od-do

5146

Kód UT WoS článku

000872607900001

EID výsledku v databázi Scopus

2-s2.0-85140637565