Block Advance Causes Substantial Error in Stereology Estimates of Cavalieri Volume and Disector-Based Cell Counts

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11140%2F24%3A10481752" target="_blank" >RIV/00216208:11140/24:10481752 - isvavai.cz</a>

Výsledek na webu

<a href="https://doi.org/10.1007/978-1-0716-3977-1_6" target="_blank" >https://doi.org/10.1007/978-1-0716-3977-1_6</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1007/978-1-0716-3977-1_6" target="_blank" >10.1007/978-1-0716-3977-1_6</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Block Advance Causes Substantial Error in Stereology Estimates of Cavalieri Volume and Disector-Based Cell Counts

Popis výsledku v původním jazyce

Background: Design-based (unbiased) stereology is considered best practices for quantifying morphometric parameters of stained biological structures in tissue sections. These methods use systematic-random sampling and unbiased geometric probes to ensure that estimates of stereology parameters converge on the expected (true) values as tissue sampling proceeds. A critical caveat is that the practical applications of these theoretically unbiased methods to biological tissue can confound the accuracy of stereology estimates.New Method: Here we show that calculations based on block advance, i.e., the instrument setting, on sectioning partially processed tissue can introduce substantial non-random error into stereology estimates of total region volume ((V_Ref ) ̂) by Cavalieri-point counting and total cell number ((N_Cell ) ̂) by disector-based methods.Comparison with Existing Method: We provide a proof that use of block advance produces similar error for (N_Cell ) ̂ calculated by the volume-based two-stage (NV . (V_Ref ) ̂) approach and fraction-based methods. Finally, a formula is given for removing this error from (V_Ref ) ̂ and (N_Cell ) ̂ using the final post-processing section thickness.

Název v anglickém jazyce

Block Advance Causes Substantial Error in Stereology Estimates of Cavalieri Volume and Disector-Based Cell Counts

Popis výsledku anglicky

Background: Design-based (unbiased) stereology is considered best practices for quantifying morphometric parameters of stained biological structures in tissue sections. These methods use systematic-random sampling and unbiased geometric probes to ensure that estimates of stereology parameters converge on the expected (true) values as tissue sampling proceeds. A critical caveat is that the practical applications of these theoretically unbiased methods to biological tissue can confound the accuracy of stereology estimates.New Method: Here we show that calculations based on block advance, i.e., the instrument setting, on sectioning partially processed tissue can introduce substantial non-random error into stereology estimates of total region volume ((V_Ref ) ̂) by Cavalieri-point counting and total cell number ((N_Cell ) ̂) by disector-based methods.Comparison with Existing Method: We provide a proof that use of block advance produces similar error for (N_Cell ) ̂ calculated by the volume-based two-stage (NV . (V_Ref ) ̂) approach and fraction-based methods. Finally, a formula is given for removing this error from (V_Ref ) ̂ and (N_Cell ) ̂ using the final post-processing section thickness.

Druh

O - Ostatní výsledky

CEP obor

—

OECD FORD obor

30103 - Neurosciences (including psychophysiology)

Projekt

<a href="/cs/project/EF16_019%2F0000787" target="_blank" >EF16_019/0000787: Centrum výzkumu infekčních onemocnění</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2024

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů