Comparative genomics of multi-drug resistant Escherichia coli ST131 from human, animal, and environmental sources in the Czech Republic

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11140%2F25%3A10498444" target="_blank" >RIV/00216208:11140/25:10498444 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/62156489:43210/25:43927093 RIV/62157124:16270/25:43882563 RIV/62157124:16810/25:43882563 RIV/65269705:_____/25:00082131

Výsledek na webu

<a href="https://verso.is.cuni.cz/pub/verso.fpl?fname=obd_publikace_handle&handle=I8Mj1qxCq" target="_blank" >https://verso.is.cuni.cz/pub/verso.fpl?fname=obd_publikace_handle&handle=I8Mj1qxCq</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.ecoenv.2025.118320" target="_blank" >10.1016/j.ecoenv.2025.118320</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Comparative genomics of multi-drug resistant Escherichia coli ST131 from human, animal, and environmental sources in the Czech Republic

Popis výsledku v původním jazyce

Globally, Escherichia coli is a leading cause of multiple drug-resistant (MDR) extraintestinal infections. ST131 is an MDR, pandemic sequence type that causes urinary tract and bloodstream infections in humans and companion animals, and systemic infections affecting multiple organs in poultry. ST131 has also been isolated from wastewater treatment plants and diverse wildlife sources. Studies investigating the phylogeny of ST131 from a One Health perspective are, however, limited. We performed whole-genome sequencing on 898 ST131 isolates from humans (n = 713), diverse environments (n = 139), wildlife (n = 32), food animals (n = 13), and companion animals (n = 1), sourced from the Czech Republic from 2005-2021, to identify evidence of clonal spread across different sources and to investigate carriage of genes encoding extended-spectrum beta-lactamases (ESBLs). ST131 belonging to clades C1 (262/898; 29 %) and C2 (528/898; 58.8 %) were dominant in our study. Irrespective of source or clade designation, most isolates (72.4 %) carried a blaCTX-M allele, with those residing in clade C1 carrying blaCTX-M-27, while those in clade C2 carried blaCTX-M-15. IncF plasmid sequence types were also segregated by clades, with F29:A-:B10 residing in clade A, F1:A2:B20 in clade C1, and F2:A1:B- and F31/F36:A4:B1 partitioning to clade C2. Clonal ST131 isolates (0-10 single-nucleotide polymorphisms; SNPs) were identified from humans, wild birds, and wastewater. In summary, our study showed that clonal ST131 isolates can be recovered across diverse sources in the Czech Republic, underscoring the importance of studying pandemic ExPEC lineages within a One Health context and across widespread geographic locations.

Název v anglickém jazyce

Comparative genomics of multi-drug resistant Escherichia coli ST131 from human, animal, and environmental sources in the Czech Republic

Popis výsledku anglicky

Globally, Escherichia coli is a leading cause of multiple drug-resistant (MDR) extraintestinal infections. ST131 is an MDR, pandemic sequence type that causes urinary tract and bloodstream infections in humans and companion animals, and systemic infections affecting multiple organs in poultry. ST131 has also been isolated from wastewater treatment plants and diverse wildlife sources. Studies investigating the phylogeny of ST131 from a One Health perspective are, however, limited. We performed whole-genome sequencing on 898 ST131 isolates from humans (n = 713), diverse environments (n = 139), wildlife (n = 32), food animals (n = 13), and companion animals (n = 1), sourced from the Czech Republic from 2005-2021, to identify evidence of clonal spread across different sources and to investigate carriage of genes encoding extended-spectrum beta-lactamases (ESBLs). ST131 belonging to clades C1 (262/898; 29 %) and C2 (528/898; 58.8 %) were dominant in our study. Irrespective of source or clade designation, most isolates (72.4 %) carried a blaCTX-M allele, with those residing in clade C1 carrying blaCTX-M-27, while those in clade C2 carried blaCTX-M-15. IncF plasmid sequence types were also segregated by clades, with F29:A-:B10 residing in clade A, F1:A2:B20 in clade C1, and F2:A1:B- and F31/F36:A4:B1 partitioning to clade C2. Clonal ST131 isolates (0-10 single-nucleotide polymorphisms; SNPs) were identified from humans, wild birds, and wastewater. In summary, our study showed that clonal ST131 isolates can be recovered across diverse sources in the Czech Republic, underscoring the importance of studying pandemic ExPEC lineages within a One Health context and across widespread geographic locations.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

30303 - Infectious Diseases

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2025

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Ecotoxicology and Environmental Safety

ISSN

0147-6513

e-ISSN

1090-2414

Svazek periodika

299

Číslo periodika v rámci svazku

July

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

10

Strana od-do

118320

Kód UT WoS článku

001504575400001

EID výsledku v databázi Scopus

2-s2.0-105005842691