Vliv inhibitorů proteasomu MG132, ZL3VS a AdaAhX3-L3VS na metabolismus proteinů u potkanů v sepsi

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11150%2F04%3A00002083" target="_blank" >RIV/00216208:11150/04:00002083 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Effects of proteasome inhibitors MG132, ZL3VS and AdaAhx3L3VS on protein metabolism in septic rats

Popis výsledku v původním jazyce

We evaluated the effects of proteasome inhibitors - a peptide aldehyde MG132, and two vinylsulfone inhibitors ZL3VS and AdaAhx3L3VS - on protein metabolism in sepsis. Sepsis was induced in rats by caecal ligation and puncture. Parameters of protein metabolism were measured in incubated rat skeletal muscles. Total proteolysis was determined according to the rates of tyrosine release into the medium during incubation. The ratesof protein synthesis and leucine oxidation were evaluated by incubating muslcesin medium containing L-/1-14C/leucine. In the septic muscles, all three inhibitors testedhad no effect on protein synthesis.MG132 decreased proteolysis by more then 50%, AdaAhx3L3VS by 20%, while the effect of ZL3VS was not significant. We conclude, that MG132 and AdaAhx3L3VS reversed at least partly the protein catabolism in sepsis, as they decreased the proteolysis and did not change the protein synthesis.

Název v anglickém jazyce

Effects of proteasome inhibitors MG132, ZL3VS and AdaAhx3L3VS on protein metabolism in septic rats

Popis výsledku anglicky

We evaluated the effects of proteasome inhibitors - a peptide aldehyde MG132, and two vinylsulfone inhibitors ZL3VS and AdaAhx3L3VS - on protein metabolism in sepsis. Sepsis was induced in rats by caecal ligation and puncture. Parameters of protein metabolism were measured in incubated rat skeletal muscles. Total proteolysis was determined according to the rates of tyrosine release into the medium during incubation. The ratesof protein synthesis and leucine oxidation were evaluated by incubating muslcesin medium containing L-/1-14C/leucine. In the septic muscles, all three inhibitors testedhad no effect on protein synthesis.MG132 decreased proteolysis by more then 50%, AdaAhx3L3VS by 20%, while the effect of ZL3VS was not significant. We conclude, that MG132 and AdaAhx3L3VS reversed at least partly the protein catabolism in sepsis, as they decreased the proteolysis and did not change the protein synthesis.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

FB - Endokrinologie, diabetologie, metabolismus, výživa

OECD FORD obor

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Projekt

<a href="/cs/project/GA303%2F03%2F1512" target="_blank" >GA303/03/1512: Možnosti ovlivnění proteinové bilance u proteokatabolických stavů - vliv inhibitorů proteasomů</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2004

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

International Journal of Experimental Pathology

ISSN

0959-9673

e-ISSN

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Svazek periodika

85

Číslo periodika v rámci svazku

1

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

9

Strana od-do

363-371

Kód UT WoS článku

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EID výsledku v databázi Scopus

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