Přímý účinek inhibitoru proteasomu AdaAhx3L3VS na metabolismus proteinů a aminokyselin

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11150%2F05%3A00003533" target="_blank" >RIV/00216208:11150/05:00003533 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Direct effects of proteasome inhibitor AdaAhx3L3VS on protein and amino acid metabolism in rat skeletal muscle

Popis výsledku v původním jazyce

We investigated direct effects of peptide vinylsulfone inhibitor AdaAhx3L3VS on protein and amino acids metabolism in rat skeletal muscle.M.soleus and m.extensor digitorum longus were incubated in metidum containing 30 umol/l AdaAhx3L3VS or no inhibitors.Total proteolysis was determined according to the rates of tyrosine release into the medium during incubation.The rates of leucine oxidation and protein synthesis were evaluated during incubation in medium containing L-[1-14C]leucine.Amino acid concentrations in the medium were measured using HPLC.Statistical comparisons were performed by paired t-tests.AdaAhx3L3VS decreased both proteolysis (by 20%) and protein synthesis (by 10-20%)and increased leucine oxidation.The release of a number of amino acidsinto the medium was lowered.We conclude that AdaAhx3L3VS decreased overall protein turnover in the muscle tissue.

Název v anglickém jazyce

Direct effects of proteasome inhibitor AdaAhx3L3VS on protein and amino acid metabolism in rat skeletal muscle

Popis výsledku anglicky

We investigated direct effects of peptide vinylsulfone inhibitor AdaAhx3L3VS on protein and amino acids metabolism in rat skeletal muscle.M.soleus and m.extensor digitorum longus were incubated in metidum containing 30 umol/l AdaAhx3L3VS or no inhibitors.Total proteolysis was determined according to the rates of tyrosine release into the medium during incubation.The rates of leucine oxidation and protein synthesis were evaluated during incubation in medium containing L-[1-14C]leucine.Amino acid concentrations in the medium were measured using HPLC.Statistical comparisons were performed by paired t-tests.AdaAhx3L3VS decreased both proteolysis (by 20%) and protein synthesis (by 10-20%)and increased leucine oxidation.The release of a number of amino acidsinto the medium was lowered.We conclude that AdaAhx3L3VS decreased overall protein turnover in the muscle tissue.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

ED - Fyziologie

OECD FORD obor

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Projekt

<a href="/cs/project/GA303%2F03%2F1512" target="_blank" >GA303/03/1512: Možnosti ovlivnění proteinové bilance u proteokatabolických stavů - vliv inhibitorů proteasomů</a><br>

Návaznosti

Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2005

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Physiological Research

ISSN

0862-8408

e-ISSN

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Svazek periodika

54

Číslo periodika v rámci svazku

5

Stát vydavatele periodika

CZ - Česká republika

Počet stran výsledku

7

Strana od-do

541-547

Kód UT WoS článku

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EID výsledku v databázi Scopus

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