Inaktivace aspartát aminostransferázy vyvolaná glykací. Vliv kyseliny močové

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11150%2F05%3A00004765" target="_blank" >RIV/00216208:11150/05:00004765 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00216208:11160/05:00007093

Výsledek na webu

—

DOI - Digital Object Identifier

—

Jazyk výsledku

angličtina

Název v původním jazyce

Glycation-Induced Inactivation of Aspartate Aminotransferase, Effect of Uric Acid

Popis výsledku v původním jazyce

Glycation is common posttranslational modification of proteins impairing their function, which occurs during diabetes mellitus and aging.Beside extracellular glycation of long-lived proteins, intracellular modifications of short-lived proteins by more reactive sugars like fructose are possible.The process includes free oxygen radicals (glycoxidation).In an attempt to reduce glycoxidation and formation of advanced glycation products, influence of 0.2-1.2 mM uric acid as endogenous antioxidant on glycoxidation of purified pig heart aspartate aminotransferase (AST) by 50 mM and 500 mM D-fructose in vitro was studied.Uric acid at 1.2 mM concentration reduced AST activity decrease and formation of total AGE products caused by incubation in vitro of the enzyme with sugar up to 25 days at 37 °C.The results thus support the hypothesis that uric acid has beneficial effects in controlling protein glycoxidation.The in vitro system AST-fructose proved to be a useful tool for investigation of glyca

Název v anglickém jazyce

Glycation-Induced Inactivation of Aspartate Aminotransferase, Effect of Uric Acid

Popis výsledku anglicky

Glycation is common posttranslational modification of proteins impairing their function, which occurs during diabetes mellitus and aging.Beside extracellular glycation of long-lived proteins, intracellular modifications of short-lived proteins by more reactive sugars like fructose are possible.The process includes free oxygen radicals (glycoxidation).In an attempt to reduce glycoxidation and formation of advanced glycation products, influence of 0.2-1.2 mM uric acid as endogenous antioxidant on glycoxidation of purified pig heart aspartate aminotransferase (AST) by 50 mM and 500 mM D-fructose in vitro was studied.Uric acid at 1.2 mM concentration reduced AST activity decrease and formation of total AGE products caused by incubation in vitro of the enzyme with sugar up to 25 days at 37 °C.The results thus support the hypothesis that uric acid has beneficial effects in controlling protein glycoxidation.The in vitro system AST-fructose proved to be a useful tool for investigation of glyca

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

CE - Biochemie

OECD FORD obor

—

Projekt

—

Návaznosti

S - Specificky vyzkum na vysokych skolach

Rok uplatnění

2005

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Molecular and Cellular Biochemistry

ISSN

0300-8177

e-ISSN

—

Svazek periodika

278

Číslo periodika v rámci svazku

1-2

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

8

Strana od-do

85-92

Kód UT WoS článku

—

EID výsledku v databázi Scopus

—