Tvorba AGE a enzymová aktivita na modelu glykoxidace AST D-frukrosou in vitro

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11150%2F05%3A00003537" target="_blank" >RIV/00216208:11150/05:00003537 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00216208:11160/05:00007092

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Enzyme Activity and AGE Formation in a Model of AST Glycoxidation by D-fructose in Vitro

Popis výsledku v původním jazyce

In the Non-enzymatic glycation as the chain reaction between reducing sugars and free amino groups of proteins has been shown to correlate with physiological ageing and severity of diabetes. In this paper, the effect of D-fructose as a reactive sugar onaspartate aminotransferase (AST) as a model protein was monitored by measurements of the enzyme activity and formation of fluorescent advanced glycation end products (AGEs). presence of fructose, total fluorescent AGEs concentration was significantly higher since 5th day of incubation compared to control values, respectively. Catalytic activity of AST clearly and quantitatively demonstrated functional changes in the enzyme molecule caused by structural modifications initiated by fructose, while the evaluation of AGE formation and especially that of pentosidine by fluorescence measurement was less reliable.

Název v anglickém jazyce

Enzyme Activity and AGE Formation in a Model of AST Glycoxidation by D-fructose in Vitro

Popis výsledku anglicky

In the Non-enzymatic glycation as the chain reaction between reducing sugars and free amino groups of proteins has been shown to correlate with physiological ageing and severity of diabetes. In this paper, the effect of D-fructose as a reactive sugar onaspartate aminotransferase (AST) as a model protein was monitored by measurements of the enzyme activity and formation of fluorescent advanced glycation end products (AGEs). presence of fructose, total fluorescent AGEs concentration was significantly higher since 5th day of incubation compared to control values, respectively. Catalytic activity of AST clearly and quantitatively demonstrated functional changes in the enzyme molecule caused by structural modifications initiated by fructose, while the evaluation of AGE formation and especially that of pentosidine by fluorescence measurement was less reliable.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

CE - Biochemie

OECD FORD obor

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Projekt

<a href="/cs/project/LN00B125" target="_blank" >LN00B125: Centrum pro výzkum struktury a mechanismu účinku potenciálních léčiv</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2005

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Acta Pharmaceutica

ISSN

1330-0075

e-ISSN

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Svazek periodika

55

Číslo periodika v rámci svazku

1

Stát vydavatele periodika

HR - Chorvatská republika

Počet stran výsledku

8

Strana od-do

107-114

Kód UT WoS článku

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EID výsledku v databázi Scopus

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