Valproic acid decreases the reparation capacity of irradiated MOLT-4 cells

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11150%2F12%3A10105092" target="_blank" >RIV/00216208:11150/12:10105092 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/68081707:_____/12:00389863 RIV/60162694:G44__/12:43874694

Výsledek na webu

<a href="http://www.springerlink.com/content/3101658251154034/fulltext.pdf" target="_blank" >http://www.springerlink.com/content/3101658251154034/fulltext.pdf</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1134/S0026893312010141" target="_blank" >10.1134/S0026893312010141</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Valproic acid decreases the reparation capacity of irradiated MOLT-4 cells

Popis výsledku v původním jazyce

The aim of our work was to evaluate mechanisms leading to radiosensitization of MOLT-4 leukemia cells following valproic acid (VA) treatment. Cells were pretreated with 2 mM VA for 24 h followed by irradiation with a dose of 0.5 or 1 Gy. The effect of both noxae, alone and combined, was detected 1 and 24 hours after the irradiation. Induction of apoptosis was evaluated by a flow cytometry. The extent of DNA damage was further determined by phosphorylation of histone H2AX using confocal microscopy. Changes in protein expression were identified by SDS-PAGE/immunoblotting. Two millimolar VA increased apoptosis induction after irradiation as well as phosphorylation of H2AX and provokes an increase in the level of p53 and its phosphorylation at Ser392 in 4h postirradiation. Likewise, p21 protein reached its maximal expression in 4 h after the irradiation of VA treated cells. Twenty four hours later, only the p53 phosphorylated at Ser15 was detected. At the same time, the protein mdm2 (nega

Název v anglickém jazyce

Valproic acid decreases the reparation capacity of irradiated MOLT-4 cells

Popis výsledku anglicky

The aim of our work was to evaluate mechanisms leading to radiosensitization of MOLT-4 leukemia cells following valproic acid (VA) treatment. Cells were pretreated with 2 mM VA for 24 h followed by irradiation with a dose of 0.5 or 1 Gy. The effect of both noxae, alone and combined, was detected 1 and 24 hours after the irradiation. Induction of apoptosis was evaluated by a flow cytometry. The extent of DNA damage was further determined by phosphorylation of histone H2AX using confocal microscopy. Changes in protein expression were identified by SDS-PAGE/immunoblotting. Two millimolar VA increased apoptosis induction after irradiation as well as phosphorylation of H2AX and provokes an increase in the level of p53 and its phosphorylation at Ser392 in 4h postirradiation. Likewise, p21 protein reached its maximal expression in 4 h after the irradiation of VA treated cells. Twenty four hours later, only the p53 phosphorylated at Ser15 was detected. At the same time, the protein mdm2 (nega

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

CE - Biochemie

OECD FORD obor

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Projekt

—

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2012

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Molecular Biology

ISSN

0026-8933

e-ISSN

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Svazek periodika

46

Číslo periodika v rámci svazku

1

Stát vydavatele periodika

RU - Ruská federace

Počet stran výsledku

7

Strana od-do

110-116

Kód UT WoS článku

000302960100013

EID výsledku v databázi Scopus

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